Wide-field high-resolution structured illumination solid immersion fluorescence microscopy

Lin Wang; Mark C. Pitter; Michael Geoffrey Somekh

doi:10.1364/OL.36.002794

Wide-field high-resolution structured illumination solid immersion fluorescence microscopy

Lin Wang, Mark C. Pitter, Michael Geoffrey Somekh

Research output: Journal article publication › Journal article › Academic research › peer-review

22 Citations (Scopus)

Abstract

The use of aplanatic solid immersion lenses (ASILs) made of high-refractive-index optical materials provides a route to wide-field high-resolution optical microscopy. Structured illumination microscopy (SIM) can double the spatial bandwidth of a microscope to also achieve high-resolution imaging. We investigate the combination of ASILs and SIM in fluorescence microscopy, which we call structured illumination solid immersion fluorescence microscopy (SISIM), to pursue a microscopic system with very large NA and high lateral resolution. We demonstrate that the combination can produce a wide-field high-resolution microscopic system with bandwidth corresponding to an NA of 3. Future developments of the SISIM system to make it achieve even higher resolution are proposed.

Original language	English
Pages (from-to)	2794-2796
Number of pages	3
Journal	Optics Letters
Volume	36
Issue number	15
DOIs	https://doi.org/10.1364/OL.36.002794
Publication status	Published - 1 Aug 2011
Externally published	Yes

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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10.1364/OL.36.002794

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AB - The use of aplanatic solid immersion lenses (ASILs) made of high-refractive-index optical materials provides a route to wide-field high-resolution optical microscopy. Structured illumination microscopy (SIM) can double the spatial bandwidth of a microscope to also achieve high-resolution imaging. We investigate the combination of ASILs and SIM in fluorescence microscopy, which we call structured illumination solid immersion fluorescence microscopy (SISIM), to pursue a microscopic system with very large NA and high lateral resolution. We demonstrate that the combination can produce a wide-field high-resolution microscopic system with bandwidth corresponding to an NA of 3. Future developments of the SISIM system to make it achieve even higher resolution are proposed.

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Wide-field high-resolution structured illumination solid immersion fluorescence microscopy

Abstract

ASJC Scopus subject areas

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