Abstract
In this study, a novel phase-sensitive surface plasmon resonance (SPR) setup, based on temporal modulation of a pumping beam by a photoelastic modulator, and subsequent extraction of phase information at the second and the third harmonics of the modulation frequency, has been developed to study biomolecular interactions on SPR-supporting gold. We demonstrated that the design setup provides ultra-high phase sensitivity, together with a wide dynamic range of measurements. In particular, the proposed scheme was used to study real-time interaction of biotin-protein and streptavidin-BSA complexes. We have found that the proposed technique has a detection limit as high as 2.89 × 10-7in terms of refractive index units (RIU). In terms of biosensing performance, a detection sensitivity of 1.3 nM from the streptavidin-maleimide/thiolated BSA complex binding reaction has also been demonstrated.
Original language | English |
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Pages (from-to) | 627-632 |
Number of pages | 6 |
Journal | Biosensors and Bioelectronics |
Volume | 23 |
Issue number | 5 |
DOIs | |
Publication status | Published - 15 Dec 2007 |
Externally published | Yes |
Keywords
- Biosensor
- Photoelastic modulator
- Surface plasmon resonance
ASJC Scopus subject areas
- Biotechnology
- Biophysics
- Biomedical Engineering
- Electrochemistry