TY - JOUR
T1 - Water-soluble iridium(III) complexes as multicolor probes for one-photon, two-photon and fluorescence lifetime imaging
AU - Ma, Yun
AU - Zhang, Diankun
AU - Lv, Wen
AU - Zhao, Qiang
AU - Wong, Wai Yeung
N1 - Funding Information:
We gratefully acknowledge the financial support from National Funds for Distinguished Young Scientists ( 61825503 ), National Natural Science Foundation of China ( 52073242 and 62075101 ), National Key R&D Program of China (2022YFE0104100), ITC Guangdong-Hong Kong Technology Cooperation Funding Scheme (TCFS) (GHP/038/19GD), CAS-Croucher Funding Scheme for Joint Laboratories (ZH4A), Miss Clarea Au for the Endowed Professorship in Energy (847S) and Research Institute for Smart Energy (CDAQ).
Publisher Copyright:
© 2023
PY - 2023/6/15
Y1 - 2023/6/15
N2 - Fluorescence microscopy imaging provides an indispensable way to visualize morphological details in tissue with subcellular resolution. Of the various imaging agents currently available, heavy-metal complex based probes offer a powerful tool for live cell imaging, primarily due to their excellent photophysical properties, including evident Stokes shifts, high photoluminescence efficiency and relatively long emission lifetime of phosphorescent signals. Herein, we have designed and synthesized seven water soluble cationic iridium(III) solvato complexes (1 − 7) for live cell imaging. The emission colors of these iridium(III) complexes can be tuned from green to red in PBS buffer (phosphate buffered saline). In particular, multicolor phosphorescent imaging for cytoplasm staining of living cells has been realized. More importantly, two-photon excited imaging and fluorescence lifetime imaging microscopy have also successfully been applied for complex 7.
AB - Fluorescence microscopy imaging provides an indispensable way to visualize morphological details in tissue with subcellular resolution. Of the various imaging agents currently available, heavy-metal complex based probes offer a powerful tool for live cell imaging, primarily due to their excellent photophysical properties, including evident Stokes shifts, high photoluminescence efficiency and relatively long emission lifetime of phosphorescent signals. Herein, we have designed and synthesized seven water soluble cationic iridium(III) solvato complexes (1 − 7) for live cell imaging. The emission colors of these iridium(III) complexes can be tuned from green to red in PBS buffer (phosphate buffered saline). In particular, multicolor phosphorescent imaging for cytoplasm staining of living cells has been realized. More importantly, two-photon excited imaging and fluorescence lifetime imaging microscopy have also successfully been applied for complex 7.
KW - Fluorescence lifetime imaging
KW - Phosphorescent iridium(III) complex
KW - Two-photon imaging
KW - Water solubility
UR - http://www.scopus.com/inward/record.url?scp=85151393872&partnerID=8YFLogxK
U2 - 10.1016/j.jorganchem.2023.122697
DO - 10.1016/j.jorganchem.2023.122697
M3 - Journal article
AN - SCOPUS:85151393872
SN - 0022-328X
VL - 992
JO - Journal of Organometallic Chemistry
JF - Journal of Organometallic Chemistry
M1 - 122697
ER -