TY - JOUR
T1 - Visualizing the Itch-Sensing Skin Arbors
AU - Xing, Yanyan
AU - Steele, Haley R.
AU - Hilley, Henry B.
AU - Zhu, Yuyan
AU - Lawson, Katy
AU - Niehoff, Taylor
AU - Han, Liang
N1 - Funding Information:
We thank Dr Xinzhong Dong for kindly providing the MrgprC11 CreERT2 , MrgprB4 PLAP , and Pirt GCamp3 mouse lines. We thank Dr Chip Hawkins in the Transgenic Core Facility at Johns Hopkins University School of Medicine (Baltimore, MD) and Dr Caiying Guo in the Gene Targeting and Transgenic Facility at Janelia Farm for the help to generate the transgenic mouse lines. We thank the Physiological Research laboratory at Georgia Institute of Technology for the animal care and services. The work was supported by grants from the U.S. National Institutes of Health ( NS087088 and HL141269 ) and Pfizer Aspire Dermatology Award to LH.
Funding Information:
We thank Dr Xinzhong Dong for kindly providing the MrgprC11CreERT2, MrgprB4PLAP, and PirtGCamp3 mouse lines. We thank Dr Chip Hawkins in the Transgenic Core Facility at Johns Hopkins University School of Medicine (Baltimore, MD) and Dr Caiying Guo in the Gene Targeting and Transgenic Facility at Janelia Farm for the help to generate the transgenic mouse lines. We thank the Physiological Research laboratory at Georgia Institute of Technology for the animal care and services. The work was supported by grants from the U.S. National Institutes of Health (NS087088 and HL141269) and Pfizer Aspire Dermatology Award to LH. Conceptualization: LH; Formal Analysis: YX, HRS, HBH, TN, KL, LH; Funding Acquisition: LH; Investigation: YX, HRS, HBH, TN, KL, LH; Resources: KL; Supervision: LH; Writing - Original Draft Preparation: YX, LH; Writing - Review and Editing: YX, HRS, LH
Publisher Copyright:
© 2021 The Authors
PY - 2021/5
Y1 - 2021/5
N2 - Diverse sensory neurons exhibit distinct neuronal morphologies with a variety of axon terminal arborizations subserving their functions. Because of its clinical significance, the molecular and cellular mechanisms of itch are being intensely studied. However, a complete analysis of itch-sensing terminal arborization is missing. Using an MrgprC11CreERT2 transgenic mouse line, we labeled a small subset of itch-sensing neurons that express multiple itch-related molecules including MrgprA3, MrgprC11, histamine receptor H1, IL-31 receptor, 5-hydroxytryptamine receptor 1F, natriuretic precursor peptide B, and neuromedin B. By combining sparse genetic labeling and whole-mount placental alkaline phosphatase histochemistry, we found that itch-sensing skin arbors exhibit free endings with extensive axonal branching in the superficial epidermis and large receptive fields. These results revealed the unique morphological characteristics of itch-sensing neurons and provide intriguing insights into the basic mechanisms of itch transmission.
AB - Diverse sensory neurons exhibit distinct neuronal morphologies with a variety of axon terminal arborizations subserving their functions. Because of its clinical significance, the molecular and cellular mechanisms of itch are being intensely studied. However, a complete analysis of itch-sensing terminal arborization is missing. Using an MrgprC11CreERT2 transgenic mouse line, we labeled a small subset of itch-sensing neurons that express multiple itch-related molecules including MrgprA3, MrgprC11, histamine receptor H1, IL-31 receptor, 5-hydroxytryptamine receptor 1F, natriuretic precursor peptide B, and neuromedin B. By combining sparse genetic labeling and whole-mount placental alkaline phosphatase histochemistry, we found that itch-sensing skin arbors exhibit free endings with extensive axonal branching in the superficial epidermis and large receptive fields. These results revealed the unique morphological characteristics of itch-sensing neurons and provide intriguing insights into the basic mechanisms of itch transmission.
UR - http://www.scopus.com/inward/record.url?scp=85096196760&partnerID=8YFLogxK
U2 - 10.1016/j.jid.2020.08.030
DO - 10.1016/j.jid.2020.08.030
M3 - Journal article
C2 - 33091423
AN - SCOPUS:85096196760
SN - 0022-202X
VL - 141
SP - 1308
EP - 1316
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 5
ER -