Varifocal Metalens for Optical Sectioning Fluorescence Microscopy

Yuan Luo, Cheng Hung Chu, Sunil Vyas, Hsin Yu Kuo, Yu Hsin Chia, Mu Ku Chen, Xu Shi, Takuo Tanaka, Hiroaki Misawa, Yi You Huang, Din Ping Tsai

Research output: Journal article publicationJournal articleAcademic researchpeer-review

70 Citations (Scopus)


Fluorescence microscopy with optical sectioning capabilities is extensively utilized in biological research to obtain three-dimensional structural images of volumetric samples. Tunable lenses have been applied in microscopy for axial scanning to acquire multiplane images. However, images acquired by conventional tunable lenses suffer from spherical aberration and distortions. Here, we design, fabricate, and implement a dielectric Moiré metalens for fluorescence imaging. The Moiré metalens consists of two complementary phase metasurfaces, with variable focal length, ranging from μ10 to μ125 mm at 532 nm by tuning mutual angles. In addition, a telecentric configuration using the Moiré metalens is designed for high-contrast multiplane fluorescence imaging. The performance of our system is evaluated by optically sectioned images obtained from HiLo illumination of fluorescently labeled beads, as well as ex vivo mice intestine tissue samples. The compact design of the varifocal metalens may find important applications in fluorescence microscopy and endoscopy for clinical purposes.

Original languageEnglish
Pages (from-to)5133-5142
Number of pages10
JournalNano Letters
Issue number12
Publication statusPublished - Jun 2021


  • fluorescence microscopy
  • HiLo imaging
  • metasurface
  • optical sectioning
  • telecentric configuration

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering


Dive into the research topics of 'Varifocal Metalens for Optical Sectioning Fluorescence Microscopy'. Together they form a unique fingerprint.

Cite this