Ultrasonic disruption of fungal mycelia for efficient recovery of polysaccharide-protein complexes from viscous fermentation broth of a medicinal fungus

Yi Ching Cheung, Xing Xun Liu, Wing Qiang Wang, Jianyong Wu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

13 Citations (Scopus)

Abstract

High-intensity ultrasound (US) was applied to facilitate the extraction of intracellular and extracellular polysaccharide-protein complexes (PSPs) from the viscous mycelial fermentation broth of a medicinal fungus Cordyceps sinensis Cs-HK1. The US treatment caused the disruption of fungal mycelia, a dramatic reduction of the apparent broth viscosity, and the release of intracellular products into the liquid medium. The degree of mycelium disruption and the rate of intracellular product release were dependent on US power intensity, treatment period and biomass concentration of broth. The extraction or release kinetics of total water-soluble products and PSPs (yield Y versus time t) under the effect of US was fitted closely to the Elovich model Y = Yo+ Y1ln t and parabolic model Y = Yo+ Y1to, respectively. Another interesting effect of the US treatment was a notable increase in the antioxidant cytoprotective activity of PSP against H2O2induced cell death.
Original languageEnglish
Pages (from-to)243-248
Number of pages6
JournalUltrasonics Sonochemistry
Volume22
DOIs
Publication statusPublished - 1 Jan 2015

Keywords

  • Cell disruption
  • Kinetic model
  • Medicinal fungus
  • Mycelial broth
  • Polysaccharide-protein complex
  • Ultrasound

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Acoustics and Ultrasonics
  • Radiology Nuclear Medicine and imaging
  • Medicine(all)

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