Ultrasensitive and closed-tube colorimetric loop-mediated isothermal amplification assay using carboxyl-modified gold nanoparticles

Jacky K F Wong; Shea Ping Yip; Ming Hung Thomas Lee

doi:10.1002/smll.201302348

Ultrasensitive and closed-tube colorimetric loop-mediated isothermal amplification assay using carboxyl-modified gold nanoparticles

Research output: Journal article publication › Journal article › Academic research › peer-review

Abstract

LAMP ends up in purple/red: The incorporation of 11-mercaptoundecanoic acid-modified gold nanoparticles into loop-mediated isothermal amplification (LAMP) reaction enables as few as 200 copies of a target DNA sequence to be detected by the naked eye. The color is controlled by magnesium ion-templated aggregation, and in the presence of the target, the LAMP reaction by-product pyrophosphate ion leads to particle deaggregation. KGaA, Weinheim.

Original language	English
Pages (from-to)	1495-1499
Number of pages	5
Journal	Small
Volume	10
Issue number	8
DOIs	https://doi.org/10.1002/smll.201302348
Publication status	Published - 24 Apr 2014

Keywords

colorimetric detection
DNA recognition
gold
isothermal amplification
nanoparticles

ASJC Scopus subject areas

Biomaterials
Engineering (miscellaneous)
Biotechnology
General Medicine

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10.1002/smll.201302348

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title = "Ultrasensitive and closed-tube colorimetric loop-mediated isothermal amplification assay using carboxyl-modified gold nanoparticles",

abstract = "LAMP ends up in purple/red: The incorporation of 11-mercaptoundecanoic acid-modified gold nanoparticles into loop-mediated isothermal amplification (LAMP) reaction enables as few as 200 copies of a target DNA sequence to be detected by the naked eye. The color is controlled by magnesium ion-templated aggregation, and in the presence of the target, the LAMP reaction by-product pyrophosphate ion leads to particle deaggregation. KGaA, Weinheim.",

keywords = "colorimetric detection, DNA recognition, gold, isothermal amplification, nanoparticles",

author = "Wong, \{Jacky K F\} and Yip, \{Shea Ping\} and Lee, \{Ming Hung Thomas\}",

year = "2014",

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doi = "10.1002/smll.201302348",

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journal = "Small",

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AU - Wong, Jacky K F

AU - Yip, Shea Ping

AU - Lee, Ming Hung Thomas

PY - 2014/4/24

Y1 - 2014/4/24

N2 - LAMP ends up in purple/red: The incorporation of 11-mercaptoundecanoic acid-modified gold nanoparticles into loop-mediated isothermal amplification (LAMP) reaction enables as few as 200 copies of a target DNA sequence to be detected by the naked eye. The color is controlled by magnesium ion-templated aggregation, and in the presence of the target, the LAMP reaction by-product pyrophosphate ion leads to particle deaggregation. KGaA, Weinheim.

AB - LAMP ends up in purple/red: The incorporation of 11-mercaptoundecanoic acid-modified gold nanoparticles into loop-mediated isothermal amplification (LAMP) reaction enables as few as 200 copies of a target DNA sequence to be detected by the naked eye. The color is controlled by magnesium ion-templated aggregation, and in the presence of the target, the LAMP reaction by-product pyrophosphate ion leads to particle deaggregation. KGaA, Weinheim.

KW - colorimetric detection

KW - DNA recognition

KW - gold

KW - isothermal amplification

KW - nanoparticles

UR - https://www.scopus.com/pages/publications/84898621397

U2 - 10.1002/smll.201302348

DO - 10.1002/smll.201302348

M3 - Journal article

C2 - 24623485

SN - 1613-6810

VL - 10

SP - 1495

EP - 1499

JO - Small

JF - Small

IS - 8

ER -

Ultrasensitive and closed-tube colorimetric loop-mediated isothermal amplification assay using carboxyl-modified gold nanoparticles

Abstract

Keywords

ASJC Scopus subject areas

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