Ultrasensitive and closed-tube colorimetric loop-mediated isothermal amplification assay using carboxyl-modified gold nanoparticles

Jacky K F Wong; Shea Ping Yip; Ming Hung Thomas Lee

doi:10.1002/smll.201302348

Ultrasensitive and closed-tube colorimetric loop-mediated isothermal amplification assay using carboxyl-modified gold nanoparticles

Jacky K F Wong, Shea Ping Yip, Ming Hung Thomas Lee

Research output: Journal article publication › Journal article › Academic research › peer-review

50 Citations (Scopus)

Abstract

LAMP ends up in purple/red: The incorporation of 11-mercaptoundecanoic acid-modified gold nanoparticles into loop-mediated isothermal amplification (LAMP) reaction enables as few as 200 copies of a target DNA sequence to be detected by the naked eye. The color is controlled by magnesium ion-templated aggregation, and in the presence of the target, the LAMP reaction by-product pyrophosphate ion leads to particle deaggregation. KGaA, Weinheim.

Original language	English
Pages (from-to)	1495-1499
Number of pages	5
Journal	Small
Volume	10
Issue number	8
DOIs	https://doi.org/10.1002/smll.201302348
Publication status	Published - 24 Apr 2014

Keywords

colorimetric detection
DNA recognition
gold
isothermal amplification
nanoparticles

ASJC Scopus subject areas

Biomaterials
Engineering (miscellaneous)
Biotechnology
General Medicine

More information

10.1002/smll.201302348

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abstract = "LAMP ends up in purple/red: The incorporation of 11-mercaptoundecanoic acid-modified gold nanoparticles into loop-mediated isothermal amplification (LAMP) reaction enables as few as 200 copies of a target DNA sequence to be detected by the naked eye. The color is controlled by magnesium ion-templated aggregation, and in the presence of the target, the LAMP reaction by-product pyrophosphate ion leads to particle deaggregation. KGaA, Weinheim.",

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AU - Lee, Ming Hung Thomas

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AB - LAMP ends up in purple/red: The incorporation of 11-mercaptoundecanoic acid-modified gold nanoparticles into loop-mediated isothermal amplification (LAMP) reaction enables as few as 200 copies of a target DNA sequence to be detected by the naked eye. The color is controlled by magnesium ion-templated aggregation, and in the presence of the target, the LAMP reaction by-product pyrophosphate ion leads to particle deaggregation. KGaA, Weinheim.

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KW - DNA recognition

KW - gold

KW - isothermal amplification

KW - nanoparticles

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Ultrasensitive and closed-tube colorimetric loop-mediated isothermal amplification assay using carboxyl-modified gold nanoparticles

Abstract

Keywords

ASJC Scopus subject areas

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