Ultrasensitive and closed-tube colorimetric loop-mediated isothermal amplification assay using carboxyl-modified gold nanoparticles

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33 Citations (Scopus)

Abstract

LAMP ends up in purple/red: The incorporation of 11-mercaptoundecanoic acid-modified gold nanoparticles into loop-mediated isothermal amplification (LAMP) reaction enables as few as 200 copies of a target DNA sequence to be detected by the naked eye. The color is controlled by magnesium ion-templated aggregation, and in the presence of the target, the LAMP reaction by-product pyrophosphate ion leads to particle deaggregation. KGaA, Weinheim.
Original languageEnglish
Pages (from-to)1495-1499
Number of pages5
JournalSmall
Volume10
Issue number8
DOIs
Publication statusPublished - 24 Apr 2014

Keywords

  • colorimetric detection
  • DNA recognition
  • gold
  • isothermal amplification
  • nanoparticles

ASJC Scopus subject areas

  • Biomaterials
  • Engineering (miscellaneous)
  • Biotechnology
  • Medicine(all)

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