Ultra-Stable oligonucleotide-gold and -silver nanoparticle conjugates prepared by a facile silica reinforcement method

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3 Citations (Scopus)


We report a simple method of enhancing the chemical stability of monothiol-modified oligonucleotide-gold and -silver nanoparticle conjugates by a thin silica reinforcement coating. Conventional conjugates prepared by chemisorption of monothiol-modified oligonucleotides onto nanoparticle surfaces undergo rapid aggregation in the presence of thiol-containing small molecules (e. g., dithiothreitol) due to ligand exchange reactions. When the conjugates are treated with (3-mercaptopropyl)trimethoxysilane, a thin silica layer is formed on the nanoparticle surface, thereby entrapping and reinforcing the thiol-gold/-silver linkage. These silica-modified oligonucleotide-gold and -silver nanoparticle conjugates become much more stable toward dithiothreitol as compared to the unmodified conjugates. Moreover, the silica layer significantly hinders the gold/silver core from oxidative dissolution by sodium cyanide. Importantly, the unique hybridization-induced color change property of the oligonucleotide-gold and -silver nanoparticle conjugates is preserved even under harsh condition (i. e., high concentrations of dithiothreitol). Taken together, these ultra-stable oligonucleotide-nanoparticle conjugates hold promise for new diagnostics and therapeutics.
Original languageEnglish
Pages (from-to)585-594
Number of pages10
JournalNano Research
Issue number9
Publication statusPublished - 1 Sept 2012


  • gold nanoparticle
  • Oligonucleotide-nanoparticle conjugate
  • silica reinforcement coating
  • silver nanoparticle

ASJC Scopus subject areas

  • Materials Science(all)
  • Electrical and Electronic Engineering


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