Tyrosine phosphorylation modulates store-operated calcium entry in cultured rat epididymal basal cells

Wu Lin Zuo; Jian Yang Du; Jie Hong Huang; Sheng Li; Geng Zhang; Si Liang Chen; Yechun Ruan; Christopher H.K. Cheng; Wen Liang Zhou

doi:10.1002/jcp.22429

Tyrosine phosphorylation modulates store-operated calcium entry in cultured rat epididymal basal cells

Wu Lin Zuo, Jian Yang Du, Jie Hong Huang, Sheng Li, Geng Zhang, Si Liang Chen, Yechun Ruan, Christopher H.K. Cheng, Wen Liang Zhou

Research output: Journal article publication › Journal article › Academic research › peer-review

9 Citations (Scopus)

Abstract

Store-operated calcium entry (SOCE) is essential for many cellular processes. In this study, we investigated modulation of SOCE by tyrosine phosphorylation in rat epididymal basal cells. The intracellular Ca2+([Ca2+]i) measurement showed that SOCE occurred in rat epididymal basal cells by pretreating the cells with thapsigargin (Tg), the inhibitor of sarco-endoplasmic reticulum Ca2+-ATPase. To identify the role of Ca2+channels in this response, we examined the effects of transient receptor potential canonical channel blockers 2-aminoethoxydiphenyl borate (2-APB), 1-[β-[3-(4-methoxyphenyl)pro-poxy]-4-methoxyphenethyl]-1H-imidazole hydrochloride(SKF96365), Gd3+, and non-selective cation channel blocker Ni2+respectively on SOCE and found that these blockers could inhibit the Ca2+influx to different extent. Furthermore, we studied the regulation of SOCE by tyrosine kinase pathway. The inhibitor of tyrosine kinase genistein remarkably suppressed the SOCE response, whereas sodium orthovanadate, the inhibitor of tyrosine phosphatase, greatly enhanced it. The results suggest that tyrosine kinase pathway plays a significant role in the initiation of SOCE and positively modulates SOCE in epididymal basal cells.

Original language	English
Pages (from-to)	1069-1073
Number of pages	5
Journal	Journal of Cellular Physiology
Volume	226
Issue number	4
DOIs	https://doi.org/10.1002/jcp.22429
Publication status	Published - 1 Apr 2011
Externally published	Yes

ASJC Scopus subject areas

Physiology
Clinical Biochemistry
Cell Biology

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10.1002/jcp.22429

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title = "Tyrosine phosphorylation modulates store-operated calcium entry in cultured rat epididymal basal cells",

abstract = "Store-operated calcium entry (SOCE) is essential for many cellular processes. In this study, we investigated modulation of SOCE by tyrosine phosphorylation in rat epididymal basal cells. The intracellular Ca2+([Ca2+]i) measurement showed that SOCE occurred in rat epididymal basal cells by pretreating the cells with thapsigargin (Tg), the inhibitor of sarco-endoplasmic reticulum Ca2+-ATPase. To identify the role of Ca2+channels in this response, we examined the effects of transient receptor potential canonical channel blockers 2-aminoethoxydiphenyl borate (2-APB), 1-[β-[3-(4-methoxyphenyl)pro-poxy]-4-methoxyphenethyl]-1H-imidazole hydrochloride(SKF96365), Gd3+, and non-selective cation channel blocker Ni2+respectively on SOCE and found that these blockers could inhibit the Ca2+influx to different extent. Furthermore, we studied the regulation of SOCE by tyrosine kinase pathway. The inhibitor of tyrosine kinase genistein remarkably suppressed the SOCE response, whereas sodium orthovanadate, the inhibitor of tyrosine phosphatase, greatly enhanced it. The results suggest that tyrosine kinase pathway plays a significant role in the initiation of SOCE and positively modulates SOCE in epididymal basal cells.",

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AU - Zuo, Wu Lin

AU - Du, Jian Yang

AU - Huang, Jie Hong

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AU - Zhang, Geng

AU - Chen, Si Liang

AU - Ruan, Yechun

AU - Cheng, Christopher H.K.

AU - Zhou, Wen Liang

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N2 - Store-operated calcium entry (SOCE) is essential for many cellular processes. In this study, we investigated modulation of SOCE by tyrosine phosphorylation in rat epididymal basal cells. The intracellular Ca2+([Ca2+]i) measurement showed that SOCE occurred in rat epididymal basal cells by pretreating the cells with thapsigargin (Tg), the inhibitor of sarco-endoplasmic reticulum Ca2+-ATPase. To identify the role of Ca2+channels in this response, we examined the effects of transient receptor potential canonical channel blockers 2-aminoethoxydiphenyl borate (2-APB), 1-[β-[3-(4-methoxyphenyl)pro-poxy]-4-methoxyphenethyl]-1H-imidazole hydrochloride(SKF96365), Gd3+, and non-selective cation channel blocker Ni2+respectively on SOCE and found that these blockers could inhibit the Ca2+influx to different extent. Furthermore, we studied the regulation of SOCE by tyrosine kinase pathway. The inhibitor of tyrosine kinase genistein remarkably suppressed the SOCE response, whereas sodium orthovanadate, the inhibitor of tyrosine phosphatase, greatly enhanced it. The results suggest that tyrosine kinase pathway plays a significant role in the initiation of SOCE and positively modulates SOCE in epididymal basal cells.

AB - Store-operated calcium entry (SOCE) is essential for many cellular processes. In this study, we investigated modulation of SOCE by tyrosine phosphorylation in rat epididymal basal cells. The intracellular Ca2+([Ca2+]i) measurement showed that SOCE occurred in rat epididymal basal cells by pretreating the cells with thapsigargin (Tg), the inhibitor of sarco-endoplasmic reticulum Ca2+-ATPase. To identify the role of Ca2+channels in this response, we examined the effects of transient receptor potential canonical channel blockers 2-aminoethoxydiphenyl borate (2-APB), 1-[β-[3-(4-methoxyphenyl)pro-poxy]-4-methoxyphenethyl]-1H-imidazole hydrochloride(SKF96365), Gd3+, and non-selective cation channel blocker Ni2+respectively on SOCE and found that these blockers could inhibit the Ca2+influx to different extent. Furthermore, we studied the regulation of SOCE by tyrosine kinase pathway. The inhibitor of tyrosine kinase genistein remarkably suppressed the SOCE response, whereas sodium orthovanadate, the inhibitor of tyrosine phosphatase, greatly enhanced it. The results suggest that tyrosine kinase pathway plays a significant role in the initiation of SOCE and positively modulates SOCE in epididymal basal cells.

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Tyrosine phosphorylation modulates store-operated calcium entry in cultured rat epididymal basal cells

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