Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates

Filip Kielar; Aileen Congreve; Ga Lai Law; Elizabeth J. New; David Parker; Ka Leung Wong; Pilar Castreňo; Javier De Mendoza

doi:10.1039/b803864c

Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates

Filip Kielar
, Aileen Congreve
, Ga Lai Law
, Elizabeth J. New
, David Parker
, Ka Leung Wong
, Pilar Castreňo
, Javier De Mendoza

Research output: Journal article publication › Journal article › Academic research › peer-review

90 Citations (Scopus)

Abstract

An emissive terbium complex has been conjugated to a C₁₂ chain, Lys-Arg₇, Arg₇, a tetraguanidinium cation and human serum albumin; two-photon excitation at 720 nm facilitated microscopy studies revealing cell localisation profiles with the oligo-guanidinium conjugate localising in mitochondria but causing apoptotic cell death (IC₅₀12 μM), the C₁₂-amide complex giving rise to necrotic cell death in skin fibroblasts (IC₅₀ 8 μM) and the peptide conjugates and the methyl ester generating punctuate cytosolic intracellular distributions.

Original language	English
Pages (from-to)	2435-2437
Number of pages	3
Journal	Chemical Communications
Issue number	21
DOIs	https://doi.org/10.1039/b803864c
Publication status	Published - 27 May 2008
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

More information

10.1039/b803864c

Cite this

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title = "Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates",

abstract = "An emissive terbium complex has been conjugated to a C12 chain, Lys-Arg7, Arg7, a tetraguanidinium cation and human serum albumin; two-photon excitation at 720 nm facilitated microscopy studies revealing cell localisation profiles with the oligo-guanidinium conjugate localising in mitochondria but causing apoptotic cell death (IC5012 μM), the C12-amide complex giving rise to necrotic cell death in skin fibroblasts (IC50 8 μM) and the peptide conjugates and the methyl ester generating punctuate cytosolic intracellular distributions.",

author = "Filip Kielar and Aileen Congreve and Law, \{Ga Lai\} and New, \{Elizabeth J.\} and David Parker and Wong, \{Ka Leung\} and Pilar Castre{\v n}o and \{De Mendoza\}, Javier",

year = "2008",

month = may,

day = "27",

doi = "10.1039/b803864c",

language = "English",

pages = "2435--2437",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "21",

}

Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates. / Kielar, Filip; Congreve, Aileen; Law, Ga Lai et al.
In: Chemical Communications, No. 21, 27.05.2008, p. 2435-2437.

Research output: Journal article publication › Journal article › Academic research › peer-review

TY - JOUR

T1 - Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates

AU - Kielar, Filip

AU - Congreve, Aileen

AU - Law, Ga Lai

AU - New, Elizabeth J.

AU - Parker, David

AU - Wong, Ka Leung

AU - Castreňo, Pilar

AU - De Mendoza, Javier

PY - 2008/5/27

Y1 - 2008/5/27

N2 - An emissive terbium complex has been conjugated to a C12 chain, Lys-Arg7, Arg7, a tetraguanidinium cation and human serum albumin; two-photon excitation at 720 nm facilitated microscopy studies revealing cell localisation profiles with the oligo-guanidinium conjugate localising in mitochondria but causing apoptotic cell death (IC5012 μM), the C12-amide complex giving rise to necrotic cell death in skin fibroblasts (IC50 8 μM) and the peptide conjugates and the methyl ester generating punctuate cytosolic intracellular distributions.

AB - An emissive terbium complex has been conjugated to a C12 chain, Lys-Arg7, Arg7, a tetraguanidinium cation and human serum albumin; two-photon excitation at 720 nm facilitated microscopy studies revealing cell localisation profiles with the oligo-guanidinium conjugate localising in mitochondria but causing apoptotic cell death (IC5012 μM), the C12-amide complex giving rise to necrotic cell death in skin fibroblasts (IC50 8 μM) and the peptide conjugates and the methyl ester generating punctuate cytosolic intracellular distributions.

UR - https://www.scopus.com/pages/publications/44049103229

U2 - 10.1039/b803864c

DO - 10.1039/b803864c

M3 - Journal article

C2 - 18491006

SN - 1359-7345

SP - 2435

EP - 2437

JO - Chemical Communications

JF - Chemical Communications

IS - 21

ER -

Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates

Abstract

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