Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates

Filip Kielar; Aileen Congreve; Ga Lai Law; Elizabeth J. New; David Parker; Ka Leung Wong; Pilar Castreňo; Javier De Mendoza

doi:10.1039/b803864c

Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates

Filip Kielar, Aileen Congreve, Ga Lai Law, Elizabeth J. New, David Parker, Ka Leung Wong, Pilar Castreňo, Javier De Mendoza

Research output: Journal article publication › Journal article › Academic research › peer-review

85 Citations (Scopus)

Abstract

An emissive terbium complex has been conjugated to a C12 chain, Lys-Arg7, Arg7, a tetraguanidinium cation and human serum albumin; two-photon excitation at 720 nm facilitated microscopy studies revealing cell localisation profiles with the oligo-guanidinium conjugate localising in mitochondria but causing apoptotic cell death (IC5012 μM), the C12-amide complex giving rise to necrotic cell death in skin fibroblasts (IC50 8 μM) and the peptide conjugates and the methyl ester generating punctuate cytosolic intracellular distributions.

Original language	English
Pages (from-to)	2435-2437
Number of pages	3
Journal	Chemical Communications
Issue number	21
DOIs	https://doi.org/10.1039/b803864c
Publication status	Published - 27 May 2008
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/b803864c

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title = "Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates",

abstract = "An emissive terbium complex has been conjugated to a C12 chain, Lys-Arg7, Arg7, a tetraguanidinium cation and human serum albumin; two-photon excitation at 720 nm facilitated microscopy studies revealing cell localisation profiles with the oligo-guanidinium conjugate localising in mitochondria but causing apoptotic cell death (IC5012 μM), the C12-amide complex giving rise to necrotic cell death in skin fibroblasts (IC50 8 μM) and the peptide conjugates and the methyl ester generating punctuate cytosolic intracellular distributions.",

author = "Filip Kielar and Aileen Congreve and Law, {Ga Lai} and New, {Elizabeth J.} and David Parker and Wong, {Ka Leung} and Pilar Castre{\v n}o and {De Mendoza}, Javier",

year = "2008",

month = may,

day = "27",

doi = "10.1039/b803864c",

language = "English",

pages = "2435--2437",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

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Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates. / Kielar, Filip; Congreve, Aileen; Law, Ga Lai et al.

In: Chemical Communications, No. 21, 27.05.2008, p. 2435-2437.

Research output: Journal article publication › Journal article › Academic research › peer-review

TY - JOUR

T1 - Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates

AU - Kielar, Filip

AU - Congreve, Aileen

AU - Law, Ga Lai

AU - New, Elizabeth J.

AU - Parker, David

AU - Wong, Ka Leung

AU - Castreňo, Pilar

AU - De Mendoza, Javier

PY - 2008/5/27

Y1 - 2008/5/27

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AB - An emissive terbium complex has been conjugated to a C12 chain, Lys-Arg7, Arg7, a tetraguanidinium cation and human serum albumin; two-photon excitation at 720 nm facilitated microscopy studies revealing cell localisation profiles with the oligo-guanidinium conjugate localising in mitochondria but causing apoptotic cell death (IC5012 μM), the C12-amide complex giving rise to necrotic cell death in skin fibroblasts (IC50 8 μM) and the peptide conjugates and the methyl ester generating punctuate cytosolic intracellular distributions.

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U2 - 10.1039/b803864c

DO - 10.1039/b803864c

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JO - Chemical Communications

JF - Chemical Communications

IS - 21

ER -

Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates

Abstract

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