Two-photon fluorescent turn-on probes for highly efficient detection and profiling of thiols in live cells and tissues

Congzhen Shen; Duoteng Zhang; Fang Xu; Yang Yang; Yi Tan; Qian Zhao; Lin Li; Ke Ding; Zhengqiu Li

doi:10.1515/hsz-2021-0189

Two-photon fluorescent turn-on probes for highly efficient detection and profiling of thiols in live cells and tissues

Congzhen Shen, Duoteng Zhang, Fang Xu, Yang Yang, Yi Tan, Qian Zhao, Lin Li, Ke Ding, Zhengqiu Li

Research output: Journal article publication › Journal article › Academic research › peer-review

1 Citation (Scopus)

Abstract

Thiols are important units in amino acids such as cysteine and peptides like glutathione. Development of chemical sensors capable of precise detection of thiols is important in cancer diagnosis and therapy. We have developed novel two-photon fluorescent turn-on probes for selective detection of thiols. The probes displayed excellent sensitivity and low detection limits. The dual-purpose probes have been demonstrated to be suitable for simultaneous imaging and proteome profiling in live cells and tumor tissues. The unique turn-on design endows the probes with excellent selectivity toward thiols in vitro and in situ, and can be further developed to support a thiol-quantification assay.

Original language	English
Pages (from-to)	445-451
Number of pages	7
Journal	Biological Chemistry
Volume	403
Issue number	4
DOIs	https://doi.org/10.1515/hsz-2021-0189
Publication status	Published - 1 Mar 2022

Keywords

cysteine residue
fluorescence probe
proteome profiling
thiol group
two-photon probes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Clinical Biochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1515/hsz-2021-0189

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title = "Two-photon fluorescent turn-on probes for highly efficient detection and profiling of thiols in live cells and tissues",

abstract = "Thiols are important units in amino acids such as cysteine and peptides like glutathione. Development of chemical sensors capable of precise detection of thiols is important in cancer diagnosis and therapy. We have developed novel two-photon fluorescent turn-on probes for selective detection of thiols. The probes displayed excellent sensitivity and low detection limits. The dual-purpose probes have been demonstrated to be suitable for simultaneous imaging and proteome profiling in live cells and tumor tissues. The unique turn-on design endows the probes with excellent selectivity toward thiols in vitro and in situ, and can be further developed to support a thiol-quantification assay.",

keywords = "cysteine residue, fluorescence probe, proteome profiling, thiol group, two-photon probes",

author = "Congzhen Shen and Duoteng Zhang and Fang Xu and Yang Yang and Yi Tan and Qian Zhao and Lin Li and Ke Ding and Zhengqiu Li",

note = "Funding Information: Funding was provided by the National Key R&D Program of China (2019YFC1711000), National Natural Science Foundation of China (21907037, 22077051, 81803389, 81820108029). RGC/CRF Equipment Grant (RGC/CRF C5033-19E), RGC/ECS (25301518). Funding Information: Research funding: Funding was provided by the National Key R&D Program of China (2019YFC1711000), National Natural Science Foundation of China (21907037, 22077051, 81803389, 81820108029). RGC/CRF Equipment Grant (RGC/CRF C5033-19E), RGC/ECS (25301518). Publisher Copyright: {\textcopyright} 2021 Walter de Gruyter GmbH, Berlin/Boston.",

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doi = "10.1515/hsz-2021-0189",

language = "English",

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T1 - Two-photon fluorescent turn-on probes for highly efficient detection and profiling of thiols in live cells and tissues

AU - Shen, Congzhen

AU - Zhang, Duoteng

AU - Xu, Fang

AU - Yang, Yang

AU - Tan, Yi

AU - Zhao, Qian

AU - Li, Lin

AU - Ding, Ke

AU - Li, Zhengqiu

N1 - Funding Information: Funding was provided by the National Key R&D Program of China (2019YFC1711000), National Natural Science Foundation of China (21907037, 22077051, 81803389, 81820108029). RGC/CRF Equipment Grant (RGC/CRF C5033-19E), RGC/ECS (25301518). Funding Information: Research funding: Funding was provided by the National Key R&D Program of China (2019YFC1711000), National Natural Science Foundation of China (21907037, 22077051, 81803389, 81820108029). RGC/CRF Equipment Grant (RGC/CRF C5033-19E), RGC/ECS (25301518). Publisher Copyright: © 2021 Walter de Gruyter GmbH, Berlin/Boston.

PY - 2022/3/1

Y1 - 2022/3/1

N2 - Thiols are important units in amino acids such as cysteine and peptides like glutathione. Development of chemical sensors capable of precise detection of thiols is important in cancer diagnosis and therapy. We have developed novel two-photon fluorescent turn-on probes for selective detection of thiols. The probes displayed excellent sensitivity and low detection limits. The dual-purpose probes have been demonstrated to be suitable for simultaneous imaging and proteome profiling in live cells and tumor tissues. The unique turn-on design endows the probes with excellent selectivity toward thiols in vitro and in situ, and can be further developed to support a thiol-quantification assay.

AB - Thiols are important units in amino acids such as cysteine and peptides like glutathione. Development of chemical sensors capable of precise detection of thiols is important in cancer diagnosis and therapy. We have developed novel two-photon fluorescent turn-on probes for selective detection of thiols. The probes displayed excellent sensitivity and low detection limits. The dual-purpose probes have been demonstrated to be suitable for simultaneous imaging and proteome profiling in live cells and tumor tissues. The unique turn-on design endows the probes with excellent selectivity toward thiols in vitro and in situ, and can be further developed to support a thiol-quantification assay.

KW - cysteine residue

KW - fluorescence probe

KW - proteome profiling

KW - thiol group

KW - two-photon probes

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Two-photon fluorescent turn-on probes for highly efficient detection and profiling of thiols in live cells and tissues

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Keywords

ASJC Scopus subject areas

UN SDGs

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