"Two-in-one" gel for spot matching after two-dimensional electrophoresis

Yuen Yuen Wang, Pik Yuen Cheung, Man Sau Wong, Chun Lap Samuel Lo

Research output: Journal article publicationJournal articleAcademic researchpeer-review

6 Citations (Scopus)

Abstract

Two-dimensional electrophoresis (2-DE) is one of the most commonly used techniques in proteomic investigations. However, due to the complex interplay of incidence including significant biological sample variations, lengthy steps involved in performing 2-DE as well as exposure time with silver staining, it is sometimes difficult to differentiate authentic differences caused by drug treatment with those artifacts caused by sample variations, running conditions of 2-DE as well as treatment time in silver staining etc. If we can compare pooled samples of control and treatment groups run in a single gel and stained together, we would be more comfortable with our findings. We propose here a low cost and highly effective method for locating differentially expressed proteins before and after drug treatment. This "two-in-one gel" technique might partially solve the problems mentioned above.
Original languageEnglish
Pages (from-to)580-583
Number of pages4
JournalProteomics
Volume3
Issue number5
DOIs
Publication statusPublished - 1 May 2003

Keywords

  • Two-in-one gel

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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