Two-dimensional biosensor arrays based on surface plasmon resonance phase imaging

C. L. Wong, H. P. Ho, T. T. Yu, Y. K. Suen, Winnie W.Y. Chow, S. Y. Wu, Wing Cheung Law, W. Yuan, W. J. Li, S. K. Kong, Chinlon Lin

Research output: Journal article publicationJournal articleAcademic researchpeer-review

38 Citations (Scopus)

Abstract

We present a biosensor design based on capturing the two-dimenstional (2D) phase image of surface plasmon resonance (SPR). This 2D SPR imaging technique may enable parallel label-free detection of multiple analytes and is compatible with the microarray chip platform. This system uses our previously reported differential phase measurement approach, in which 2D phase maps obtained from the signal (P) and reference (S) polarizations are compared pixel by pixel. This technique greatly improves detection resolution as the subtraction step can eliminate measurement fluctuations caused by external disturbances as they essentially appear in both channels. Unlike conventional angular SPR systems, in which illumination from a range of angles must be used, phase measurement requires illumination from only one angle, thus making it well suited for 2D measurement. Also, phase-stepping introduced from a moving mirror provides the necessary modulation for accurate detection of the phase. In light of the rapidly increasing need for fast real-time detection, quantification, and identification of a range of proteins for various biomedical applications, our 2D SPR phase imaging technique should hold a promising future in the medical device market.
Original languageEnglish
Pages (from-to)2325-2332
Number of pages8
JournalApplied Optics
Volume46
Issue number12
DOIs
Publication statusPublished - 20 Apr 2007
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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