TY - JOUR
T1 - Tuning the unsaturated iron sites in MIL-101(Fe) nanoparticles for reactive oxygen species-mediated bacterial inactivation in the dark
AU - Peng, Shiqi
AU - Li, Rong
AU - Rao, Yongfang
AU - Huang, Yu
AU - Zhao, Yulei
AU - Xiong, Mingyu
AU - Cao, Junji
AU - Lee, Shuncheng
PY - 2022/11/5
Y1 - 2022/11/5
N2 - In this study, reactive oxygen species (ROS)-mediated antibacterial activity with high efficiency in the dark was achieved by modulating the active sites of MIL-101(Fe) nanoparticles. The ROS production over x-MIL-101(Fe) nanoparticles was intensively enhanced by tuning the unsaturated iron sites (FeII/FeIII, the ratio was marked as x%). Electron paramagnetic resonance (EPR) analysis confirmedmore ROS generation on x-MIL-101(Fe) surface than that on MIL-101(Fe), due to more electrons shifting from benzene rings to FeII/FeIII sites. Among all samples, 8.9-MIL-101(Fe) displayed the highest inactivation efficiency (> 99.99%) against Escherichia coli within 2 h in the dark. ROS reacted with cell wall components to generate carbon-centered radicals via H abstraction, leading to the disruption of cell wall, intracellular ROS, and DNA damage. Antibacterial performance of 8.9-MIL-101(Fe) in air filters indicated 8.9-MIL-101(Fe) can be applied to prevent the spread of airborne pathogens. The results are promising for the ambient antibacterial application of MOF materials.
AB - In this study, reactive oxygen species (ROS)-mediated antibacterial activity with high efficiency in the dark was achieved by modulating the active sites of MIL-101(Fe) nanoparticles. The ROS production over x-MIL-101(Fe) nanoparticles was intensively enhanced by tuning the unsaturated iron sites (FeII/FeIII, the ratio was marked as x%). Electron paramagnetic resonance (EPR) analysis confirmedmore ROS generation on x-MIL-101(Fe) surface than that on MIL-101(Fe), due to more electrons shifting from benzene rings to FeII/FeIII sites. Among all samples, 8.9-MIL-101(Fe) displayed the highest inactivation efficiency (> 99.99%) against Escherichia coli within 2 h in the dark. ROS reacted with cell wall components to generate carbon-centered radicals via H abstraction, leading to the disruption of cell wall, intracellular ROS, and DNA damage. Antibacterial performance of 8.9-MIL-101(Fe) in air filters indicated 8.9-MIL-101(Fe) can be applied to prevent the spread of airborne pathogens. The results are promising for the ambient antibacterial application of MOF materials.
KW - MIL-101(Fe) nanoparticles
KW - Unsaturated iron sites
KW - Reactive oxygen species
KW - DNA strand breakage
KW - Bacteria inactivation
U2 - 10.1016/j.apcatb.2022.121693
DO - 10.1016/j.apcatb.2022.121693
M3 - Journal article
SN - 0926-3373
VL - 316
JO - Applied Catalysis B: Environmental
JF - Applied Catalysis B: Environmental
M1 - 121693
ER -