Tumorigenic cell selection and substrate rigidity manipulation using 2D and 3D extracellular matrices

Juan Zhang; Wentao Ha; Yuhan Chang; Yutong Ge; Xiaowei Wei; Youhua Tan; Qiong Jia

doi:10.1016/j.xpro.2022.101577

Tumorigenic cell selection and substrate rigidity manipulation using 2D and 3D extracellular matrices

Juan Zhang, Wentao Ha, Yuhan Chang, Yutong Ge, Xiaowei Wei, Youhua Tan, Qiong Jia

Research output: Journal article publication › Journal article › Academic research › peer-review

1 Citation (Scopus)

Abstract

Soft fibrin gels are used to select tumorigenic cells and regulate the stemness and metastasis of colorectal cancer cells via mechanotransduction. This protocol details steps to produce two-dimensional (2D) and three-dimensional (3D) extracellular matrices for substrate rigidity manipulation and tumorigenic cell selection. We also describe how it can be applied to tumor mechanotransductive research by colony growth monitoring and cell isolation. For complete details on the use and execution of this protocol, please refer to Chang et al. (2022).

Original language	English
Article number	101577
Journal	STAR Protocols
Volume	3
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2022.101577
Publication status	Published - 16 Sept 2022

Keywords

Biophysics
Biotechnology and bioengineering
Cancer
Cell Biology

ASJC Scopus subject areas

General Neuroscience
General Biochemistry,Genetics and Molecular Biology
General Immunology and Microbiology

More information

10.1016/j.xpro.2022.101577

http://hdl.handle.net/10397/101648

Cite this

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title = "Tumorigenic cell selection and substrate rigidity manipulation using 2D and 3D extracellular matrices",

abstract = "Soft fibrin gels are used to select tumorigenic cells and regulate the stemness and metastasis of colorectal cancer cells via mechanotransduction. This protocol details steps to produce two-dimensional (2D) and three-dimensional (3D) extracellular matrices for substrate rigidity manipulation and tumorigenic cell selection. We also describe how it can be applied to tumor mechanotransductive research by colony growth monitoring and cell isolation. For complete details on the use and execution of this protocol, please refer to Chang et al. (2022).",

keywords = "Biophysics, Biotechnology and bioengineering, Cancer, Cell Biology",

author = "Juan Zhang and Wentao Ha and Yuhan Chang and Yutong Ge and Xiaowei Wei and Youhua Tan and Qiong Jia",

note = "Funding Information: This work was supported by grants from the National Natural Science Foundation of China (no. 82103576 ), and the Natural Science Foundation of Jiangsu Province (no. BK20200146 ) to Q.J. Publisher Copyright: {\textcopyright} 2022 The Author(s)",

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T1 - Tumorigenic cell selection and substrate rigidity manipulation using 2D and 3D extracellular matrices

AU - Zhang, Juan

AU - Ha, Wentao

AU - Chang, Yuhan

AU - Ge, Yutong

AU - Wei, Xiaowei

AU - Tan, Youhua

AU - Jia, Qiong

N1 - Funding Information: This work was supported by grants from the National Natural Science Foundation of China (no. 82103576 ), and the Natural Science Foundation of Jiangsu Province (no. BK20200146 ) to Q.J. Publisher Copyright: © 2022 The Author(s)

PY - 2022/9/16

Y1 - 2022/9/16

N2 - Soft fibrin gels are used to select tumorigenic cells and regulate the stemness and metastasis of colorectal cancer cells via mechanotransduction. This protocol details steps to produce two-dimensional (2D) and three-dimensional (3D) extracellular matrices for substrate rigidity manipulation and tumorigenic cell selection. We also describe how it can be applied to tumor mechanotransductive research by colony growth monitoring and cell isolation. For complete details on the use and execution of this protocol, please refer to Chang et al. (2022).

AB - Soft fibrin gels are used to select tumorigenic cells and regulate the stemness and metastasis of colorectal cancer cells via mechanotransduction. This protocol details steps to produce two-dimensional (2D) and three-dimensional (3D) extracellular matrices for substrate rigidity manipulation and tumorigenic cell selection. We also describe how it can be applied to tumor mechanotransductive research by colony growth monitoring and cell isolation. For complete details on the use and execution of this protocol, please refer to Chang et al. (2022).

KW - Biophysics

KW - Biotechnology and bioengineering

KW - Cancer

KW - Cell Biology

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DO - 10.1016/j.xpro.2022.101577

M3 - Journal article

C2 - 35880125

AN - SCOPUS:85134750357

SN - 2666-1667

VL - 3

JO - STAR Protocols

JF - STAR Protocols

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M1 - 101577

ER -

Tumorigenic cell selection and substrate rigidity manipulation using 2D and 3D extracellular matrices

Abstract

Keywords

ASJC Scopus subject areas

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