Transposon-tagged mutagenesis in the rat

Kazuhiro Kitada; Satoshi Ishishita; Keiko Tosaka; Ri Ichi Takahashi; Masatsugu Ueda; Wee-Keong Vincent Keng; Kyoji Horie; Junji Takeda

doi:10.1038/nmeth1002

Transposon-tagged mutagenesis in the rat

Kazuhiro Kitada, Satoshi Ishishita, Keiko Tosaka, Ri Ichi Takahashi, Masatsugu Ueda, Wee-Keong Vincent Keng, Kyoji Horie, Junji Takeda

Research output: Journal article publication › Journal article › Academic research › peer-review

86 Citations (Scopus)

Abstract

Although the laboratory rat (Rattus norvegicus) is an indispensable experimental animal for biomedical research and drug development, the lack of embryonic stem cell lines hampers gene-knockout studies. Here we report the successful generation of insertional mutant rats using the Sleeping Beauty (SB) transposon system. This would benefit a variety of biomedical research fields for which the rat model is better suited than the mouse model.

Original language	English
Pages (from-to)	131-133
Number of pages	3
Journal	Nature Methods
Volume	4
Issue number	2
DOIs	https://doi.org/10.1038/nmeth1002
Publication status	Published - 1 Feb 2007
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth1002

Cite this

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AU - Ueda, Masatsugu

AU - Keng, Wee-Keong Vincent

AU - Horie, Kyoji

AU - Takeda, Junji

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Transposon-tagged mutagenesis in the rat

Abstract

ASJC Scopus subject areas

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