Transposon-tagged mutagenesis in the rat

Kazuhiro Kitada, Satoshi Ishishita, Keiko Tosaka, Ri Ichi Takahashi, Masatsugu Ueda, Wee-Keong Vincent Keng, Kyoji Horie, Junji Takeda

Research output: Journal article publicationJournal articleAcademic researchpeer-review

83 Citations (Scopus)

Abstract

Although the laboratory rat (Rattus norvegicus) is an indispensable experimental animal for biomedical research and drug development, the lack of embryonic stem cell lines hampers gene-knockout studies. Here we report the successful generation of insertional mutant rats using the Sleeping Beauty (SB) transposon system. This would benefit a variety of biomedical research fields for which the rat model is better suited than the mouse model.
Original languageEnglish
Pages (from-to)131-133
Number of pages3
JournalNature Methods
Volume4
Issue number2
DOIs
Publication statusPublished - 1 Feb 2007
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this