Transposon-tagged mutagenesis in the rat

Kazuhiro Kitada, Satoshi Ishishita, Keiko Tosaka, Ri Ichi Takahashi, Masatsugu Ueda, Wee-Keong Vincent Keng, Kyoji Horie, Junji Takeda

Research output: Journal article publicationJournal articleAcademic researchpeer-review

86 Citations (Scopus)


Although the laboratory rat (Rattus norvegicus) is an indispensable experimental animal for biomedical research and drug development, the lack of embryonic stem cell lines hampers gene-knockout studies. Here we report the successful generation of insertional mutant rats using the Sleeping Beauty (SB) transposon system. This would benefit a variety of biomedical research fields for which the rat model is better suited than the mouse model.
Original languageEnglish
Pages (from-to)131-133
Number of pages3
JournalNature Methods
Issue number2
Publication statusPublished - 1 Feb 2007
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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