Transmission Near-Field Scanning Optical Microscopy Investigation on Cellular Uptake Behavior of Iron Oxide Nanoparticles

Yu Zhang, Jennifer Reiber Kyle, Miro Penchev, Vahid Yazdanpanah, Jinjiang Yu, Yi Li, Mo Yang, Gurer Budak, Ekmel Ozbay, Mihrimah Ozkan, Cengiz S. Ozkan

Research output: Journal article publicationJournal articleAcademic researchpeer-review

3 Citations (Scopus)


Cellular uptake behavior of iron oxide nanoparticles is investigated using a transmission near-field scanning optical microscopy (NSOM) without the need of fluorescent labeling. By using the transmission NSOM system, we could simultaneously explore the near-field optical analysis of the cell interior and record the topographic information of the cell surface. The cell endocytosis of iron oxide nanoparticles by normal breast MCF10A cells is first studied by this transmission NSOM system, and this dual functional nanoscale-resolution microscopy shows the capability of mapping the spatial localization of nanoparticles in/outside cell surface without the need of fluorescence labeling. Nanoscale optical signature patterns for iron oxide nanoparticle-loaded vesicles inside the cells were observed and analyzed.
Original languageEnglish
Pages (from-to)135-143
Number of pages9
Issue number3
Publication statusPublished - 1 Dec 2012


  • AFM
  • Endocytosis
  • Iron oxide
  • MCF10A
  • Nanoparticle
  • NSOM

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

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