Transcriptional profiling of the chick retina identifies down-regulation of VIP and UTS2B genes during early lens-induced myopia

Sze Wan Shan, Pan Feng Wang, Jimmy Ka Wai Cheung, Fengjuan Yu, Hui Zheng, Shumeng Luo, Shea Ping Yip, Chi Ho To, Thomas Chuen Lam

Research output: Journal article publicationJournal articleAcademic researchpeer-review

Abstract

Gene expression of the chick retina was examined during the early development of lens-induced
myopia (LIM) using whole transcriptome sequencing. Monocular treatment of the right eyes with 10
diopter (D) lenses was performed on newly born chicks for one day (LIM-24) or two days (LIM-48), while the contralateral eyes without lenses served as controls. Myopia development was confirmed by demonstrating significant elongation of the optical axis in lens-treated eyes compared to untreated control eyes. RNA was extracted and RNA-seq was performed using the Illumina HiSeqTM 2000 platform. Data analysis was carried out on a Parteks Flow platform. Using screening criteria of Z1.30- fold change and a false discovery rate o1%, 11 (five down-regulated and six up-regulated) and 35 differentially expressed genes (six down-regulated and twenty-nine up-regulated) were identified at 24 hours and 48 hours, respectively. Using another cohort for validation, Quantitative PCR confirmed significant changes in the expression of VIP and UTS2B mRNA (P o0.05) after only 24 hours of LIM treatment and numerical changes in the expression for PCGF5 and FOXG1, which were consistent with transcriptome sequencing but did not reach statistical significance. These data suggest that concerted changes of retinal gene expression may be instrumental in the initiation of axial elongation and myopia development.
Original languageEnglish
JournalMol. Omics
DOIs
Publication statusPublished - Mar 2022

Cite this