Towards automating micro cellular detection process using micro vortex pump arrays

Raymond H.W. Lam, Kin Fong Lei, Lei Miao, Zaili Dong, Wing Cheung Law, Yick Keung Suen, Wen J. Li, Aaron Ho Pu Ho, Siu Kai Kong

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review

Abstract

This paper reports a polymer based microfluidic analysis system integrated with a surface plasmon resonance (SPR) biosensor for detecting the specific binding of biomolecules and qualitatively monitoring of cell adhesion on the sensor surface. Micropumps, microchannels, and SPR biosensor were integrated into a single polymer (PMMA) based microfluidic system. The integrated system has been studied in its potential application in bio-molecules detection and drug discovery. Two experiments, 1) monitoring the reaction between BSA-BSA antibody, and 2) monitoring the activities of living cells in the presence or absence of trypsin in RPMI-1640 medium, were conducted to show the feasibility of real-time cellular and molecular detection. Based on these successful experimental results we have also developed a computer-controllable vortex micropump system that will eventually automate many bio-molecular detection and drug discovery processes.
Original languageEnglish
Title of host publicationIEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM
Pages66-71
Number of pages6
Publication statusPublished - 16 Nov 2005
Externally publishedYes
EventProceedings of the 2005 IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM 2005 - Monterey, CA, United States
Duration: 24 Jul 200528 Jul 2005

Conference

ConferenceProceedings of the 2005 IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM 2005
CountryUnited States
CityMonterey, CA
Period24/07/0528/07/05

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Software
  • Computer Science Applications
  • Electrical and Electronic Engineering

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