Towards automating micro cellular detection process using micro vortex pump arrays

Raymond H.W. Lam; Kin Fong Lei; Lei Miao; Zaili Dong; Wing Cheung Law; Yick Keung Suen; Wen J. Li; Aaron Ho Pu Ho; Siu Kai Kong

Towards automating micro cellular detection process using micro vortex pump arrays

Raymond H.W. Lam, Kin Fong Lei, Lei Miao, Zaili Dong, Wing Cheung Law, Yick Keung Suen, Wen J. Li, Aaron Ho Pu Ho, Siu Kai Kong

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

Abstract

This paper reports a polymer based microfluidic analysis system integrated with a surface plasmon resonance (SPR) biosensor for detecting the specific binding of biomolecules and qualitatively monitoring of cell adhesion on the sensor surface. Micropumps, microchannels, and SPR biosensor were integrated into a single polymer (PMMA) based microfluidic system. The integrated system has been studied in its potential application in bio-molecules detection and drug discovery. Two experiments, 1) monitoring the reaction between BSA-BSA antibody, and 2) monitoring the activities of living cells in the presence or absence of trypsin in RPMI-1640 medium, were conducted to show the feasibility of real-time cellular and molecular detection. Based on these successful experimental results we have also developed a computer-controllable vortex micropump system that will eventually automate many bio-molecular detection and drug discovery processes.

Original language	English
Title of host publication	IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM
Pages	66-71
Number of pages	6
Publication status	Published - 16 Nov 2005
Externally published	Yes
Event	Proceedings of the 2005 IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM 2005 - Monterey, CA, United States Duration: 24 Jul 2005 → 28 Jul 2005

Conference

Conference	Proceedings of the 2005 IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM 2005
Country/Territory	United States
City	Monterey, CA
Period	24/07/05 → 28/07/05

ASJC Scopus subject areas

Control and Systems Engineering
Software
Computer Science Applications
Electrical and Electronic Engineering

Cite this

@inproceedings{20be1db6fa2948e48e73fabc0d3edf6f,

title = "Towards automating micro cellular detection process using micro vortex pump arrays",

abstract = "This paper reports a polymer based microfluidic analysis system integrated with a surface plasmon resonance (SPR) biosensor for detecting the specific binding of biomolecules and qualitatively monitoring of cell adhesion on the sensor surface. Micropumps, microchannels, and SPR biosensor were integrated into a single polymer (PMMA) based microfluidic system. The integrated system has been studied in its potential application in bio-molecules detection and drug discovery. Two experiments, 1) monitoring the reaction between BSA-BSA antibody, and 2) monitoring the activities of living cells in the presence or absence of trypsin in RPMI-1640 medium, were conducted to show the feasibility of real-time cellular and molecular detection. Based on these successful experimental results we have also developed a computer-controllable vortex micropump system that will eventually automate many bio-molecular detection and drug discovery processes.",

author = "Lam, {Raymond H.W.} and Lei, {Kin Fong} and Lei Miao and Zaili Dong and Law, {Wing Cheung} and Suen, {Yick Keung} and Li, {Wen J.} and Ho, {Aaron Ho Pu} and Kong, {Siu Kai}",

year = "2005",

month = nov,

day = "16",

language = "English",

pages = "66--71",

booktitle = "IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM",

note = "Proceedings of the 2005 IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM 2005 ; Conference date: 24-07-2005 Through 28-07-2005",

}

Lam, RHW, Lei, KF, Miao, L, Dong, Z, Law, WC, Suen, YK, Li, WJ, Ho, AHP & Kong, SK 2005, Towards automating micro cellular detection process using micro vortex pump arrays. in IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM., MA3-04, pp. 66-71, Proceedings of the 2005 IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM 2005, Monterey, CA, United States, 24/07/05.

Towards automating micro cellular detection process using micro vortex pump arrays. / Lam, Raymond H.W.; Lei, Kin Fong; Miao, Lei et al.
IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM. 2005. p. 66-71 MA3-04.

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

TY - GEN

T1 - Towards automating micro cellular detection process using micro vortex pump arrays

AU - Lam, Raymond H.W.

AU - Lei, Kin Fong

AU - Miao, Lei

AU - Dong, Zaili

AU - Law, Wing Cheung

AU - Suen, Yick Keung

AU - Li, Wen J.

AU - Ho, Aaron Ho Pu

AU - Kong, Siu Kai

PY - 2005/11/16

Y1 - 2005/11/16

N2 - This paper reports a polymer based microfluidic analysis system integrated with a surface plasmon resonance (SPR) biosensor for detecting the specific binding of biomolecules and qualitatively monitoring of cell adhesion on the sensor surface. Micropumps, microchannels, and SPR biosensor were integrated into a single polymer (PMMA) based microfluidic system. The integrated system has been studied in its potential application in bio-molecules detection and drug discovery. Two experiments, 1) monitoring the reaction between BSA-BSA antibody, and 2) monitoring the activities of living cells in the presence or absence of trypsin in RPMI-1640 medium, were conducted to show the feasibility of real-time cellular and molecular detection. Based on these successful experimental results we have also developed a computer-controllable vortex micropump system that will eventually automate many bio-molecular detection and drug discovery processes.

AB - This paper reports a polymer based microfluidic analysis system integrated with a surface plasmon resonance (SPR) biosensor for detecting the specific binding of biomolecules and qualitatively monitoring of cell adhesion on the sensor surface. Micropumps, microchannels, and SPR biosensor were integrated into a single polymer (PMMA) based microfluidic system. The integrated system has been studied in its potential application in bio-molecules detection and drug discovery. Two experiments, 1) monitoring the reaction between BSA-BSA antibody, and 2) monitoring the activities of living cells in the presence or absence of trypsin in RPMI-1640 medium, were conducted to show the feasibility of real-time cellular and molecular detection. Based on these successful experimental results we have also developed a computer-controllable vortex micropump system that will eventually automate many bio-molecular detection and drug discovery processes.

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M3 - Conference article published in proceeding or book

SP - 66

EP - 71

BT - IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM

T2 - Proceedings of the 2005 IEEE/ASME International Conference on Advanced Intelligent Mechatronics, AIM 2005

Y2 - 24 July 2005 through 28 July 2005

ER -

Towards automating micro cellular detection process using micro vortex pump arrays

Abstract

Conference

ASJC Scopus subject areas

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