TY - JOUR
T1 - Tilapia dsRNA-activated protein kinase R (PKR): An interferon-induced antiviral effector with translation inhibition activity
AU - Gan, Zhen
AU - Cheng, Jun
AU - Hou, Jing
AU - Chen, Shannan
AU - Xia, Hongli
AU - Xia, Liqun
AU - Kwok, Kevin W.H.
AU - Lu, Yishan
AU - Nie, Pin
N1 - Funding Information:
We thank all the laboratory members for their critical reviews and comments on this manuscript. This work was financially supported by the grants from the National Key Research and Development Project ( 2020YFD0900201 ), the China Agricultural Research System ( CARS-46 ), and the Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams ( 2019KJ141 ), and also by a project from the China Postdoctoral Science Foundation ( 2020M682529 ).
Publisher Copyright:
© 2021
PY - 2021/5
Y1 - 2021/5
N2 - The dsRNA-activated protein kinase R (PKR) is one of key antiviral effectors induced by interferons (IFNs), and its functions are largely unknown in tilapia, an important commercial fish species suffering from several viral infectious diseases. In the present study, a PKR gene named On-PKR was identified and cloned from Nile tilapia, Oreochromis niloticus. On-PKR gene was constitutively expressed in all tissues examined, with the highest expression level observed in head kidney and liver, and was rapidly induced in all organs/tissues tested following the stimulation of poly(I:C). Importantly, the expression of On-PKR is induced by group I and group II IFNs with distinct induction kinetics in vivo: group I IFN elicits a relative delayed but sustained induction of On-PKR, whereas group II IFN triggers a rapid and transient expression of On-PKR. Moreover, the overexpression of On-PKR has been proven to inhibit the protein translation and virus replication in fish cells. The present study thus contributes to a better understanding of the functions of antiviral effectors in tilapia, and may provide clues for the prevention and therapy of viral diseases in fish.
AB - The dsRNA-activated protein kinase R (PKR) is one of key antiviral effectors induced by interferons (IFNs), and its functions are largely unknown in tilapia, an important commercial fish species suffering from several viral infectious diseases. In the present study, a PKR gene named On-PKR was identified and cloned from Nile tilapia, Oreochromis niloticus. On-PKR gene was constitutively expressed in all tissues examined, with the highest expression level observed in head kidney and liver, and was rapidly induced in all organs/tissues tested following the stimulation of poly(I:C). Importantly, the expression of On-PKR is induced by group I and group II IFNs with distinct induction kinetics in vivo: group I IFN elicits a relative delayed but sustained induction of On-PKR, whereas group II IFN triggers a rapid and transient expression of On-PKR. Moreover, the overexpression of On-PKR has been proven to inhibit the protein translation and virus replication in fish cells. The present study thus contributes to a better understanding of the functions of antiviral effectors in tilapia, and may provide clues for the prevention and therapy of viral diseases in fish.
KW - Antiviral activity
KW - Oreochromis niloticus
KW - PKR
KW - Translation inhibition activity
KW - Type I interferons
UR - http://www.scopus.com/inward/record.url?scp=85101958296&partnerID=8YFLogxK
U2 - 10.1016/j.fsi.2021.02.016
DO - 10.1016/j.fsi.2021.02.016
M3 - Journal article
C2 - 33667675
AN - SCOPUS:85101958296
SN - 1050-4648
VL - 112
SP - 74
EP - 80
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
ER -
