TY - JOUR
T1 - The toxic effects of microcystin-LR on mouse lungs and alveolar type II epithelial cells
AU - Wang, Cong
AU - Gu, Shen
AU - Yin, Xiaoqin
AU - Yuan, Mingming
AU - Zou, Xiang
AU - Li, Zutong
AU - Cao, Honghui
AU - Meng, Xiannan
AU - Hu, Kebin
AU - Han, Xiaodong
PY - 2016/6/1
Y1 - 2016/6/1
N2 - However, the effect of MC-LR on lung remains unclear. In this study, we investigated the chronic, low-dose effect of MC-LR on mouse lung tissues and the influence of MC-LR on mouse alveolar type II epithelial cells (ATII cells). Methods MC-LR was orally administered to mice at 0, 1, 10, and 40 μg/L for 6 consecutive months and mouse lungs were obtained for histopathological and immunoblot analysis. ATII cells were cultured in various concentrations of MC-LR (0, 0.5, 5, 50, 500 nmol/L) for indicated time and the cell viability and proteins change were tested. Results Our study revealed that the chronic, low-dose MC-LR exposure induced alveolar collapse and lung cell apoptosis as well as the breach of cell junction integrity. Furthermore, following treatment with MC-LR, ATII cells could uptake MC-LR, resulting in apoptosis and disruption of cell junction integrity. Conclusions These data support the toxic potential of low-dose MC-LR in rendering chronic injury to lung tissues.
AB - However, the effect of MC-LR on lung remains unclear. In this study, we investigated the chronic, low-dose effect of MC-LR on mouse lung tissues and the influence of MC-LR on mouse alveolar type II epithelial cells (ATII cells). Methods MC-LR was orally administered to mice at 0, 1, 10, and 40 μg/L for 6 consecutive months and mouse lungs were obtained for histopathological and immunoblot analysis. ATII cells were cultured in various concentrations of MC-LR (0, 0.5, 5, 50, 500 nmol/L) for indicated time and the cell viability and proteins change were tested. Results Our study revealed that the chronic, low-dose MC-LR exposure induced alveolar collapse and lung cell apoptosis as well as the breach of cell junction integrity. Furthermore, following treatment with MC-LR, ATII cells could uptake MC-LR, resulting in apoptosis and disruption of cell junction integrity. Conclusions These data support the toxic potential of low-dose MC-LR in rendering chronic injury to lung tissues.
KW - Chronic low dose
KW - Lung
KW - Microcystin-LR
KW - Toxic
UR - http://www.scopus.com/inward/record.url?scp=85008600064&partnerID=8YFLogxK
U2 - 10.1016/j.toxicon.2016.03.007
DO - 10.1016/j.toxicon.2016.03.007
M3 - Journal article
C2 - 26995211
SN - 0041-0101
VL - 115
SP - 81
EP - 88
JO - Toxicon
JF - Toxicon
ER -