The Small Open Reading Frame-Encoded Peptides: Advances in Methodologies and Functional Studies

Lei Chen; Ying Yang; Yuanliang Zhang; Kecheng Li; Hongmin Cai; Hongwei Wang; Qian Zhao

doi:10.1002/cbic.202100534

The Small Open Reading Frame-Encoded Peptides: Advances in Methodologies and Functional Studies

Lei Chen
, Ying Yang
, Yuanliang Zhang
, Kecheng Li
, Hongmin Cai
, Hongwei Wang
, Qian Zhao

Research output: Journal article publication › Review article › Academic research › peer-review

6 Citations (Scopus)

Abstract

Small open reading frames (sORFs) are an important class of genes with less than 100 codons. They were historically annotated as noncoding or even junk sequences. In recent years, accumulating evidence suggests that sORFs could encode a considerable number of polypeptides, many of which play important roles in both physiology and disease pathology. However, it has been technically challenging to directly detect sORF-encoded peptides (SEPs). Here, we discuss the latest advances in methodologies for identifying SEPs with mass spectrometry, as well as the progress on functional studies of SEPs.

Original language	English
Article number	e202100534
Journal	ChemBioChem
Volume	23
Issue number	8
DOIs	https://doi.org/10.1002/cbic.202100534
Publication status	Published - 20 Apr 2022

Keywords

mass spectrometry
proteins
proteomics
small open reading frames
sORF-encoded peptides

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

More information

10.1002/cbic.202100534

http://hdl.handle.net/10397/95716

Cite this

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title = "The Small Open Reading Frame-Encoded Peptides: Advances in Methodologies and Functional Studies",

abstract = "Small open reading frames (sORFs) are an important class of genes with less than 100 codons. They were historically annotated as noncoding or even junk sequences. In recent years, accumulating evidence suggests that sORFs could encode a considerable number of polypeptides, many of which play important roles in both physiology and disease pathology. However, it has been technically challenging to directly detect sORF-encoded peptides (SEPs). Here, we discuss the latest advances in methodologies for identifying SEPs with mass spectrometry, as well as the progress on functional studies of SEPs.",

keywords = "mass spectrometry, proteins, proteomics, small open reading frames, sORF-encoded peptides",

author = "Lei Chen and Ying Yang and Yuanliang Zhang and Kecheng Li and Hongmin Cai and Hongwei Wang and Qian Zhao",

note = "Funding Information: Our work on sORFs is funded by Research Grants Council (RGC) ECS 25301518, GRF 15305821 and RGC-CRF Equipment C5033-19E. We also acknowledge the funding support from Laboratory for Synthetic Chemistry and Chemical Biology under the Health@InnoHK Program launched by Innovation and Technology Commission, The Government of Hong Kong Special Administrative Region of the People's Republic of China. Funding Information: Our work on sORFs is funded by Research Grants Council (RGC) ECS 25301518, GRF 15305821 and RGC‐CRF Equipment C5033‐19E. We also acknowledge the funding support from Laboratory for Synthetic Chemistry and Chemical Biology under the Health@InnoHK Program launched by Innovation and Technology Commission, The Government of Hong Kong Special Administrative Region of the People's Republic of China. Publisher Copyright: {\textcopyright} 2021 Wiley-VCH GmbH.",

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day = "20",

doi = "10.1002/cbic.202100534",

language = "English",

volume = "23",

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AU - Chen, Lei

AU - Yang, Ying

AU - Zhang, Yuanliang

AU - Li, Kecheng

AU - Cai, Hongmin

AU - Wang, Hongwei

AU - Zhao, Qian

N1 - Funding Information: Our work on sORFs is funded by Research Grants Council (RGC) ECS 25301518, GRF 15305821 and RGC-CRF Equipment C5033-19E. We also acknowledge the funding support from Laboratory for Synthetic Chemistry and Chemical Biology under the Health@InnoHK Program launched by Innovation and Technology Commission, The Government of Hong Kong Special Administrative Region of the People's Republic of China. Funding Information: Our work on sORFs is funded by Research Grants Council (RGC) ECS 25301518, GRF 15305821 and RGC‐CRF Equipment C5033‐19E. We also acknowledge the funding support from Laboratory for Synthetic Chemistry and Chemical Biology under the Health@InnoHK Program launched by Innovation and Technology Commission, The Government of Hong Kong Special Administrative Region of the People's Republic of China. Publisher Copyright: © 2021 Wiley-VCH GmbH.

PY - 2022/4/20

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N2 - Small open reading frames (sORFs) are an important class of genes with less than 100 codons. They were historically annotated as noncoding or even junk sequences. In recent years, accumulating evidence suggests that sORFs could encode a considerable number of polypeptides, many of which play important roles in both physiology and disease pathology. However, it has been technically challenging to directly detect sORF-encoded peptides (SEPs). Here, we discuss the latest advances in methodologies for identifying SEPs with mass spectrometry, as well as the progress on functional studies of SEPs.

AB - Small open reading frames (sORFs) are an important class of genes with less than 100 codons. They were historically annotated as noncoding or even junk sequences. In recent years, accumulating evidence suggests that sORFs could encode a considerable number of polypeptides, many of which play important roles in both physiology and disease pathology. However, it has been technically challenging to directly detect sORF-encoded peptides (SEPs). Here, we discuss the latest advances in methodologies for identifying SEPs with mass spectrometry, as well as the progress on functional studies of SEPs.

KW - mass spectrometry

KW - proteins

KW - proteomics

KW - small open reading frames

KW - sORF-encoded peptides

UR - https://www.scopus.com/pages/publications/85120682139

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M3 - Review article

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AN - SCOPUS:85120682139

SN - 1439-4227

VL - 23

JO - ChemBioChem

JF - ChemBioChem

IS - 8

M1 - e202100534

ER -

The Small Open Reading Frame-Encoded Peptides: Advances in Methodologies and Functional Studies

Abstract

Keywords

ASJC Scopus subject areas

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