The Small Open Reading Frame-Encoded Peptides: Advances in Methodologies and Functional Studies

Lei Chen, Ying Yang, Yuanliang Zhang, Kecheng Li, Hongmin Cai, Hongwei Wang, Qian Zhao

Research output: Journal article publicationReview articleAcademic researchpeer-review

5 Citations (Scopus)

Abstract

Small open reading frames (sORFs) are an important class of genes with less than 100 codons. They were historically annotated as noncoding or even junk sequences. In recent years, accumulating evidence suggests that sORFs could encode a considerable number of polypeptides, many of which play important roles in both physiology and disease pathology. However, it has been technically challenging to directly detect sORF-encoded peptides (SEPs). Here, we discuss the latest advances in methodologies for identifying SEPs with mass spectrometry, as well as the progress on functional studies of SEPs.

Original languageEnglish
Article numbere202100534
JournalChemBioChem
Volume23
Issue number8
DOIs
Publication statusPublished - 20 Apr 2022

Keywords

  • mass spectrometry
  • proteins
  • proteomics
  • small open reading frames
  • sORF-encoded peptides

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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