Abstract
PURPOSE: To study the saturation characteristics of the glucose transport across the bovine retinal pigment epithelium(RPE). METHODS: The bovine RPE preparations were munted with a modified Ussing chamber. The L-[3H]-glucose and 3-O-methyl-D-[14C]-glucose fluxes across the RPE from the choroid to retina were studied at different glucose concentrations. RESULTS: The glucose transport was found to be stereospecific, with 3-O-methyl-D-glucose (MDG) being transported about three times faster than L-glucose. The glucose transport showed typical saturation characteristics in Michaelis-Menten fashion. The Vmax and the Km of corrected MDG were 2452 nmol cm-2h-1 and 30.8 mM respectively. It was shown that the glucose transport system was saturated at 61.6 mM. CONCLUSIONS: The saturation characteristics of the corrected MDG flux suggested that the capacity of glucose transport through the bovine RPE is immense.
| Original language | English |
|---|---|
| Pages (from-to) | 126-129 |
| Number of pages | 4 |
| Journal | Yan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu |
| Volume | 14 |
| Issue number | 3 |
| Publication status | Published - 1 Sept 1998 |
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems