The saturation characteristics of glucose transport in bovine retinal pigment epithelium.

Chi Ho To, K. K. Cheung, S. H. Chiu, H. M. Lai, K. S. Lung

Research output: Journal article publicationJournal articleAcademic researchpeer-review

5 Citations (Scopus)

Abstract

PURPOSE: To study the saturation characteristics of the glucose transport across the bovine retinal pigment epithelium(RPE). METHODS: The bovine RPE preparations were munted with a modified Ussing chamber. The L-[3H]-glucose and 3-O-methyl-D-[14C]-glucose fluxes across the RPE from the choroid to retina were studied at different glucose concentrations. RESULTS: The glucose transport was found to be stereospecific, with 3-O-methyl-D-glucose (MDG) being transported about three times faster than L-glucose. The glucose transport showed typical saturation characteristics in Michaelis-Menten fashion. The Vmax and the Km of corrected MDG were 2452 nmol cm-2h-1 and 30.8 mM respectively. It was shown that the glucose transport system was saturated at 61.6 mM. CONCLUSIONS: The saturation characteristics of the corrected MDG flux suggested that the capacity of glucose transport through the bovine RPE is immense.
Original languageEnglish
Pages (from-to)126-129
Number of pages4
JournalYan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu
Volume14
Issue number3
Publication statusPublished - 1 Sep 1998

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

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