In the present study, increasing ethylenediaminetetraacetic acid (EDTA) concentration from 0 to 0.5 mmol L-1resulted in progressive increases in root elongation and in shoot and root dry matter (DM) of Indian mustard seedlings (Brassica juncea. L.) exposed at 0.5 mmol L-1of lead (Pb). The highest concentration of Pb in the shoots of Indian mustard reached 1140 mg kg-1dry weight (DW) in the treatment with 0.5 mmol L-1of Pb + 0.25 mmol L-1of EDTA. A significantly positive correlation was found between the concentrations of Pb and EDTA in the shoots of mustard. Roots were pretreated with an MC (methanol:trichloromethane) solution, 0.1 mol L-1of HCl, and 65°C hot water. The plants were then exposed to 0.5 mmol L-1of Pb + 3 mmol L-1of EDTA in solution for 2 d. The pretreatments with MC, HCl, and hot water all increased the concentration of Pb in shoots by 14-, 7-, and 15-fold, respectively, compared with the shoots that had not been pretreated. Therefore, some physiological damage to roots would be useful to enhance the uptake of metal by plants and to minimize the application of doses of chelates in the practical operation of chelate-assisted phytoremediation.
- Ethylenediaminetetraacetic acid (EDTA)
- Lead (Pb)
- Root damage
ASJC Scopus subject areas
- Plant Science