The osteoprotective effect of Herba epimedii (HEP) extract in vivo and in vitro

Fang Xie, Chun Fu Wu, Wan Ping Lai, Xu Juan Yang, Pik Yuan Cheung, Xin Sheng Yao, Ping Chung Leung, Man Sau Wong

Research output: Journal article publicationJournal articleAcademic researchpeer-review

100 Citations (Scopus)

Abstract

Herba epimedii (HEP) is one of the most frequently used herbs prescribed for treatment of osteoporosis in China. In the present study, the in vivo effects of HEP extract on bone metabolism were evaluated using 4-month-old ovariectomized (OVX) or sham-operated (Sham) female Sprague-Dawley rats orally administered with HEP extract (110 mg kg-1d-1), 17β-estrogen (2 mg kg-1d-1) or its vehicle for 3 months. HEP extract significantly decreased urinary calcium excretion, suppressed serum alkaline phosphatase (ALP) activity and urinary deoxypyridinoline levels in OVX rats (P < 0.05 versus vehicle-treated OVX rats). Histomorphometric analysis indicated that HEP extract could prevent OVX-induced bone loss by increasing tibial trabecular bone area and decreasing trabecular separation in OVX rats (P < 0.05 versus vehicle-treated OVX group). The in vitro effects of HEP extract were also studied using rat osteoblast-like UMR 106 cells. HEP extract significantly stimulated cell proliferation in a dose-dependent manner (P < 0.01 versus vehicle-treated) and increased ALP activity at 200 μg ml-1 (P <0.01 versus vehicle-treated) in UMR 106 cells. It modulated osteoclastogenesis by increasing osteoprotegrin (OPG) mRNA and decreasing receptor activator of NF-κ ligand (RANKL) mRNA expression, resulting in a dose-dependent increase in OPG/RANKL mRNA ratio (P < 0.01 versus vehicle-treated). Taken together, HEP treatment can effectively suppress the OVX-induced increase in bone turnover possibly by both an increase in osteoblastic activities and a decrease in osteoclastogenesis. The present study provides the evidence that HEP can be considered as a complementary and alternative medicine for treatment of postmenopausal osteoporosis.
Original languageEnglish
Pages (from-to)353-361
Number of pages9
JournalEvidence-based Complementary and Alternative Medicine
Volume2
Issue number3
DOIs
Publication statusPublished - 1 Sep 2005

Keywords

  • Herba Epimedium
  • Osteoblast-like UMR 106 cells
  • Osteoclastogenesis
  • Osteoporosis
  • Ovariectomized rat

ASJC Scopus subject areas

  • Complementary and alternative medicine

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