TY - JOUR
T1 - The non-invasive diagnosis of colorectal cancer via a SOX9-based gene panel
AU - Xue, Vivian Weiwen
AU - Ng, Simon Siu Man
AU - Tsang, Hin Fung
AU - Wong, Heong Ting
AU - Leung, Wing Wa
AU - Wong, Yee Ni
AU - Wong, Yin Kwan Evelyn
AU - Yu, Allen Chi Shing
AU - Yim, Aldrin Kay Yuen
AU - Cho, William Chi Shing
AU - Tai, William Chi Shing
AU - Wong, Sze Chuen Cesar
N1 - Funding Information:
This work was supported by Research Grants Council HK and HK Innovation and Technology Fund University-Industry Collaborative Programme (RGCQ71P and UIM/354), and National Natural Science Foundation of China (82103438).
Publisher Copyright:
© 2023, The Author(s), under exclusive licence to Springer Nature Switzerland AG.
PY - 2023
Y1 - 2023
N2 - Colorectal cancer (CRC) threatens human health seriously. Early diagnosis of CRC is critical to improving patient survival. Meanwhile, non-invasive detection through tumor-circulating markers can be an important auxiliary diagnosis. In this study, we performed targeted RNA sequencing in paired tumor and adjacent normal fresh frozen tissues from 68 patients, and we also measured circulating mRNA levels in 4 time-point plasma samples collected before and after operation or chemotherapy. Our results showed that SOX9 (6.73-fold with adjusted p value < 1 × 10–45), MYC (20.59-fold with adjusted p value < 1 × 10–57), and MMP7 (131.94-fold with adjusted p value < 1 × 10–78) highly expressed in tumor compared with adjacent normal tissues. Besides, the circulating mRNA of SOX9 (41.14-fold with adjusted p value < 1 × 10–13) in CRC was significantly higher than in the normal control as well. Moreover, a SOX9-based 9-gene panel (SOX9, GSK3A, FZD4, LEF1, DVL1, FZD7, NFATC1, KRT19, and RUVBL1) showed the non-invasive diagnostic value of CRC (AUC: 0.863 (0.766–0.960), TPR: 0.92, TNR: 0.87). In summary, SOX9 expression consistently increases in tumor and plasma samples from CRC patients, which indicates the important role of SOX9 in CRC progression and its potential in non-invasive diagnosis of CRC.
AB - Colorectal cancer (CRC) threatens human health seriously. Early diagnosis of CRC is critical to improving patient survival. Meanwhile, non-invasive detection through tumor-circulating markers can be an important auxiliary diagnosis. In this study, we performed targeted RNA sequencing in paired tumor and adjacent normal fresh frozen tissues from 68 patients, and we also measured circulating mRNA levels in 4 time-point plasma samples collected before and after operation or chemotherapy. Our results showed that SOX9 (6.73-fold with adjusted p value < 1 × 10–45), MYC (20.59-fold with adjusted p value < 1 × 10–57), and MMP7 (131.94-fold with adjusted p value < 1 × 10–78) highly expressed in tumor compared with adjacent normal tissues. Besides, the circulating mRNA of SOX9 (41.14-fold with adjusted p value < 1 × 10–13) in CRC was significantly higher than in the normal control as well. Moreover, a SOX9-based 9-gene panel (SOX9, GSK3A, FZD4, LEF1, DVL1, FZD7, NFATC1, KRT19, and RUVBL1) showed the non-invasive diagnostic value of CRC (AUC: 0.863 (0.766–0.960), TPR: 0.92, TNR: 0.87). In summary, SOX9 expression consistently increases in tumor and plasma samples from CRC patients, which indicates the important role of SOX9 in CRC progression and its potential in non-invasive diagnosis of CRC.
KW - Circulating mRNA
KW - Colorectal cancer
KW - Non-invasive diagnosis
KW - Targeted RNA sequencing
UR - http://www.scopus.com/inward/record.url?scp=85146220008&partnerID=8YFLogxK
U2 - 10.1007/s10238-022-00970-6
DO - 10.1007/s10238-022-00970-6
M3 - Journal article
C2 - 36637582
AN - SCOPUS:85146220008
SN - 1591-8890
JO - Clinical and Experimental Medicine
JF - Clinical and Experimental Medicine
ER -