The Effect of Low-Dose Atropine on Alpha Ganglion Cell Signaling in the Mouse Retina

Qin Wang; Seema Banerjee; Chung Him So; Chun Ting Qiu; Ying Hon Sze; Thomas Chuen Lam; Chi-Ho To; Feng Pan

doi:10.3389/fncel.2021.664491

The Effect of Low-Dose Atropine on Alpha Ganglion Cell Signaling in the Mouse Retina

Qin Wang, Seema Banerjee, Chung Him So, Chun Ting Qiu, Ying Hon Sze, Thomas Chuen Lam, Chi-Ho To, Feng Pan

Research output: Journal article publication › Journal article › Academic research › peer-review

8 Citations (Scopus)

Abstract

Low-dose atropine helps to control myopia progression with few side effects. However, the impact of atropine, a non-selective muscarinic Acetylcholine (ACh) receptor antagonist, on retinal ganglion cells (RGCs) remains unclear. After immersing the cornea and adjacent conjunctiva of enucleated eyes in 0.05% (approximately 800 μM) atropine solution for 30 min, the atropine concentration reached in the retina was below 2 μM. After direct superfusion of the retina with 1 μM atropine (considering that the clinical application of 0.05% atropine eye drops will be diluted over time due to tear flow for 30 min), no noticeable changes in the morphology of ON and OFF alpha RGCs (αRGCs) were observed. Atropine affected the light-evoked responses of ON and OFF αRGCs in a dose- and time-dependent fashion. Direct application of less than 100 μM atropine on the retina did not affect light-evoked responses. The time latency of light-induced responses of ON or OFF αRGCs did not change after the application of 0.05–100 μM atropine for 5 min. However, 50 μM atropine extended the threshold of joint inter-spike interval (ISI) distribution of the RGCs. These results indicated that low-dose atropine (

Original language	English
Article number	664491
Journal	Frontiers in Cellular Neuroscience
Volume	15
DOIs	https://doi.org/10.3389/fncel.2021.664491
Publication status	Published - 5 May 2021

Keywords

atropine
circuit
myopia
retina
retinal ganglion cells (RGCs)
vision

ASJC Scopus subject areas

Cellular and Molecular Neuroscience

More information

10.3389/fncel.2021.664491

Cite this

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title = "The Effect of Low-Dose Atropine on Alpha Ganglion Cell Signaling in the Mouse Retina",

abstract = "Low-dose atropine helps to control myopia progression with few side effects. However, the impact of atropine, a non-selective muscarinic Acetylcholine (ACh) receptor antagonist, on retinal ganglion cells (RGCs) remains unclear. After immersing the cornea and adjacent conjunctiva of enucleated eyes in 0.05% (approximately 800 μM) atropine solution for 30 min, the atropine concentration reached in the retina was below 2 μM. After direct superfusion of the retina with 1 μM atropine (considering that the clinical application of 0.05% atropine eye drops will be diluted over time due to tear flow for 30 min), no noticeable changes in the morphology of ON and OFF alpha RGCs (αRGCs) were observed. Atropine affected the light-evoked responses of ON and OFF αRGCs in a dose- and time-dependent fashion. Direct application of less than 100 μM atropine on the retina did not affect light-evoked responses. The time latency of light-induced responses of ON or OFF αRGCs did not change after the application of 0.05–100 μM atropine for 5 min. However, 50 μM atropine extended the threshold of joint inter-spike interval (ISI) distribution of the RGCs. These results indicated that low-dose atropine (",

keywords = "atropine, circuit, myopia, retina, retinal ganglion cells (RGCs), vision",

author = "Qin Wang and Seema Banerjee and So, {Chung Him} and Qiu, {Chun Ting} and Sze, {Ying Hon} and Lam, {Thomas Chuen} and Chi-Ho To and Feng Pan",

note = "Funding Information: This study was supported by the Hong Kong Polytechnic University (grants: UAG4, UAHA, ZE1G, and ZVN2), Henry G. Leong Endowed Professorship in Elderly Vision Health (8-8475), and the University Research Facility in Behavioral and Systems Neuroscience. This study was also supported by a grant from the Hong Kong Research Grants Council (ECS/RGC; 25103918 and 15104819; Hong Kong, SAR). Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Wang, Banerjee, So, Qiu, Sze, Lam, To and Pan.",

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AU - Wang, Qin

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AU - Sze, Ying Hon

AU - Lam, Thomas Chuen

AU - To, Chi-Ho

AU - Pan, Feng

N1 - Funding Information: This study was supported by the Hong Kong Polytechnic University (grants: UAG4, UAHA, ZE1G, and ZVN2), Henry G. Leong Endowed Professorship in Elderly Vision Health (8-8475), and the University Research Facility in Behavioral and Systems Neuroscience. This study was also supported by a grant from the Hong Kong Research Grants Council (ECS/RGC; 25103918 and 15104819; Hong Kong, SAR). Publisher Copyright: © Copyright © 2021 Wang, Banerjee, So, Qiu, Sze, Lam, To and Pan.

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N2 - Low-dose atropine helps to control myopia progression with few side effects. However, the impact of atropine, a non-selective muscarinic Acetylcholine (ACh) receptor antagonist, on retinal ganglion cells (RGCs) remains unclear. After immersing the cornea and adjacent conjunctiva of enucleated eyes in 0.05% (approximately 800 μM) atropine solution for 30 min, the atropine concentration reached in the retina was below 2 μM. After direct superfusion of the retina with 1 μM atropine (considering that the clinical application of 0.05% atropine eye drops will be diluted over time due to tear flow for 30 min), no noticeable changes in the morphology of ON and OFF alpha RGCs (αRGCs) were observed. Atropine affected the light-evoked responses of ON and OFF αRGCs in a dose- and time-dependent fashion. Direct application of less than 100 μM atropine on the retina did not affect light-evoked responses. The time latency of light-induced responses of ON or OFF αRGCs did not change after the application of 0.05–100 μM atropine for 5 min. However, 50 μM atropine extended the threshold of joint inter-spike interval (ISI) distribution of the RGCs. These results indicated that low-dose atropine (

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KW - retinal ganglion cells (RGCs)

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DO - 10.3389/fncel.2021.664491

M3 - Journal article

SN - 1662-5102

VL - 15

JO - Frontiers in Cellular Neuroscience

JF - Frontiers in Cellular Neuroscience

M1 - 664491

ER -

The Effect of Low-Dose Atropine on Alpha Ganglion Cell Signaling in the Mouse Retina

Abstract

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