The Effect of Low-Dose Atropine on Alpha Ganglion Cell Signaling in the Mouse Retina

Qin Wang, Seema Banerjee, ChungHim So, ChunTing Qiu, YingHon Sze, Thomas Chuen Lam, Chi-Ho To, Feng Pan

Research output: Journal article publicationJournal articleAcademic researchpeer-review

Abstract

Low-dose atropine helps to control myopia progression with few side effects. However, the impact of atropine, a non-selective muscarinic Acetylcholine (ACh) receptor antagonist, on retinal ganglion cells (RGCs) remains unclear. After immersing the cornea and adjacent conjunctiva of enucleated eyes in 0.05% (approximately 800 μM) atropine solution for 30 min, the atropine concentration reached in the retina was below 2 μM. After direct superfusion of the retina with 1 μM atropine (considering that the clinical application of 0.05% atropine eye drops will be diluted over time due to tear flow for 30 min), no noticeable changes in the morphology of ON and OFF alpha RGCs (αRGCs) were observed. Atropine affected the light-evoked responses of ON and OFF αRGCs in a dose- and time-dependent fashion. Direct application of less than 100 μM atropine on the retina did not affect light-evoked responses. The time latency of light-induced responses of ON or OFF αRGCs did not change after the application of 0.05–100 μM atropine for 5 min. However, 50 μM atropine extended the threshold of joint inter-spike interval (ISI) distribution of the RGCs. These results indicated that low-dose atropine (
Original languageEnglish
Article number664491
JournalFrontiers in Cellular Neuroscience
Volume15
DOIs
Publication statusPublished - 5 May 2021

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