The effect of centrifugation on circulating mRNA quantitation opens up a new scenario in expression profiling from patients with metastatic colorectal cancer

Sze Chuen Cesar Wong; Brigette B Y Ma; Paul B S Lai; Simon S M Ng; Janet F Y Lee; Edwin P. Hui; Money Y Y Lam; Charles M L Chan; Anthony T C Chan

doi:10.1016/j.clinbiochem.2007.08.006

The effect of centrifugation on circulating mRNA quantitation opens up a new scenario in expression profiling from patients with metastatic colorectal cancer

Sze Chuen Cesar Wong, Brigette B Y Ma, Paul B S Lai, Simon S M Ng, Janet F Y Lee, Edwin P. Hui, Money Y Y Lam, Charles M L Chan, Anthony T C Chan

Research output: Journal article publication › Journal article › Academic research › peer-review

9 Citations (Scopus)

Abstract

Objectives: To investigate the effect of centrifugation in circulating mRNA quantitation and to design a new, consistent protocol for it. Design and methods: The concentrations of β-catenin mRNA, P-selectin mRNA, cytokeratin 20 mRNA, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA in the plasma, the mononuclear and red cells (MARC) from metastatic colorectal (CRC) patients whose blood samples were centrifuged with 2 different forces, were measured. A new protocol to measure expression profiles in MARC was studied. Results: When the centrifugal force was increased: (1) the concentrations of all transcripts were dramatically decreased in the plasma samples; (2) only the concentrations of P-selectin mRNA and GAPDH mRNA were significantly increased in the mononuclear cell samples; and (3) their concentrations in the red cells did not change significantly. The new protocol found 9 differential expressed genes. Conclusions: Centrifugal force causes inconsistent mRNA quantitation in the plasma. The new protocol discovered 9 differential expressed genes for metastatic CRC.

Original language	English
Pages (from-to)	1277-1284
Number of pages	8
Journal	Clinical Biochemistry
Volume	40
Issue number	16-17
DOIs	https://doi.org/10.1016/j.clinbiochem.2007.08.006
Publication status	Published - 1 Nov 2007
Externally published	Yes

Keywords

Centrifugation
Expression profiling
Metastatic colorectal cancer
mRNA
Plasma
Red cells

ASJC Scopus subject areas

Clinical Biochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

More information

10.1016/j.clinbiochem.2007.08.006

Cite this

Wong, S. C. C., Ma, B. B. Y., Lai, P. B. S., Ng, S. S. M., Lee, J. F. Y., Hui, E. P., Lam, M. Y. Y., Chan, C. M. L., & Chan, A. T. C. (2007). The effect of centrifugation on circulating mRNA quantitation opens up a new scenario in expression profiling from patients with metastatic colorectal cancer. Clinical Biochemistry, 40(16-17), 1277-1284. https://doi.org/10.1016/j.clinbiochem.2007.08.006

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abstract = "Objectives: To investigate the effect of centrifugation in circulating mRNA quantitation and to design a new, consistent protocol for it. Design and methods: The concentrations of β-catenin mRNA, P-selectin mRNA, cytokeratin 20 mRNA, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA in the plasma, the mononuclear and red cells (MARC) from metastatic colorectal (CRC) patients whose blood samples were centrifuged with 2 different forces, were measured. A new protocol to measure expression profiles in MARC was studied. Results: When the centrifugal force was increased: (1) the concentrations of all transcripts were dramatically decreased in the plasma samples; (2) only the concentrations of P-selectin mRNA and GAPDH mRNA were significantly increased in the mononuclear cell samples; and (3) their concentrations in the red cells did not change significantly. The new protocol found 9 differential expressed genes. Conclusions: Centrifugal force causes inconsistent mRNA quantitation in the plasma. The new protocol discovered 9 differential expressed genes for metastatic CRC.",

keywords = "Centrifugation, Expression profiling, Metastatic colorectal cancer, mRNA, Plasma, Red cells",

author = "Wong, {Sze Chuen Cesar} and Ma, {Brigette B Y} and Lai, {Paul B S} and Ng, {Simon S M} and Lee, {Janet F Y} and Hui, {Edwin P.} and Lam, {Money Y Y} and Chan, {Charles M L} and Chan, {Anthony T C}",

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doi = "10.1016/j.clinbiochem.2007.08.006",

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The effect of centrifugation on circulating mRNA quantitation opens up a new scenario in expression profiling from patients with metastatic colorectal cancer. / Wong, Sze Chuen Cesar; Ma, Brigette B Y; Lai, Paul B S et al.
In: Clinical Biochemistry, Vol. 40, No. 16-17, 01.11.2007, p. 1277-1284.

Research output: Journal article publication › Journal article › Academic research › peer-review

TY - JOUR

T1 - The effect of centrifugation on circulating mRNA quantitation opens up a new scenario in expression profiling from patients with metastatic colorectal cancer

AU - Wong, Sze Chuen Cesar

AU - Ma, Brigette B Y

AU - Lai, Paul B S

AU - Ng, Simon S M

AU - Lee, Janet F Y

AU - Hui, Edwin P.

AU - Lam, Money Y Y

AU - Chan, Charles M L

AU - Chan, Anthony T C

PY - 2007/11/1

Y1 - 2007/11/1

N2 - Objectives: To investigate the effect of centrifugation in circulating mRNA quantitation and to design a new, consistent protocol for it. Design and methods: The concentrations of β-catenin mRNA, P-selectin mRNA, cytokeratin 20 mRNA, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA in the plasma, the mononuclear and red cells (MARC) from metastatic colorectal (CRC) patients whose blood samples were centrifuged with 2 different forces, were measured. A new protocol to measure expression profiles in MARC was studied. Results: When the centrifugal force was increased: (1) the concentrations of all transcripts were dramatically decreased in the plasma samples; (2) only the concentrations of P-selectin mRNA and GAPDH mRNA were significantly increased in the mononuclear cell samples; and (3) their concentrations in the red cells did not change significantly. The new protocol found 9 differential expressed genes. Conclusions: Centrifugal force causes inconsistent mRNA quantitation in the plasma. The new protocol discovered 9 differential expressed genes for metastatic CRC.

AB - Objectives: To investigate the effect of centrifugation in circulating mRNA quantitation and to design a new, consistent protocol for it. Design and methods: The concentrations of β-catenin mRNA, P-selectin mRNA, cytokeratin 20 mRNA, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA in the plasma, the mononuclear and red cells (MARC) from metastatic colorectal (CRC) patients whose blood samples were centrifuged with 2 different forces, were measured. A new protocol to measure expression profiles in MARC was studied. Results: When the centrifugal force was increased: (1) the concentrations of all transcripts were dramatically decreased in the plasma samples; (2) only the concentrations of P-selectin mRNA and GAPDH mRNA were significantly increased in the mononuclear cell samples; and (3) their concentrations in the red cells did not change significantly. The new protocol found 9 differential expressed genes. Conclusions: Centrifugal force causes inconsistent mRNA quantitation in the plasma. The new protocol discovered 9 differential expressed genes for metastatic CRC.

KW - Centrifugation

KW - Expression profiling

KW - Metastatic colorectal cancer

KW - mRNA

KW - Plasma

KW - Red cells

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DO - 10.1016/j.clinbiochem.2007.08.006

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JO - Clinical Biochemistry

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The effect of centrifugation on circulating mRNA quantitation opens up a new scenario in expression profiling from patients with metastatic colorectal cancer

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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