TY - JOUR
T1 - The current and future applications of in situ hybridization technologies in anatomical pathology
AU - Leung, Hoi Yi
AU - Yeung, Martin Ho Yin
AU - Leung, Wai Tung
AU - Wong, King Hin
AU - Tang, Wai Yan
AU - Cho, William Chi Shing
AU - Wong, Heong Ting
AU - Tsang, Hin Fung
AU - Wong, Yin Kwan Evelyn
AU - Pei, Xiao Meng
AU - Cheng, Hennie Yuk Lin
AU - Chan, Amanda Kit Ching
AU - Wong, Sze Chuen Cesar
N1 - Funding Information:
This paper was not funded The authors thank Dr. John K.C. Chan for his invaluable comments and suggestions in this paper.
Publisher Copyright:
© 2021 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2022/1
Y1 - 2022/1
N2 - Introduction: In situ hybridization (ISH) plays an important role in the field of molecular diagnostics, especially in an anatomical pathology laboratory. ISH is a technique that can detect the targeted DNA or RNA sequences in tissue sections from frozen or fixed materials with labeled DNA or RNA probes. Radioactive and non-radioactive probes are the two major probes that can be used to label the targeted nucleic acids. Areas covered: Two decades after the Human Genome Project, ISH has not only simply been applied to identify the chromosomal location of a human gene but has also been extensively applied to gene expressions studies and utilized for clinical diagnosis, especially for the determination of biomarkers for breast and ovarian cancers–human epidermal growth factor receptor 2. Duchenne muscular dystrophy, Cri-du-chat syndrome, Angelman syndrome, PraderWilli syndrome, cystic fibrosis, and trisomy are diseases that can also be detected by ISH. In this review, the basic principles, historical development, advantages and disadvantages, enhancement in reporting molecules and probes, advancement in detection methods, in situ PCR, clinical applications and novel applications of ISH will be discussed. Expert opinion: With the advancement in ISH technologies and appropriate training, diagnosis can be improved in Anatomical Pathology.
AB - Introduction: In situ hybridization (ISH) plays an important role in the field of molecular diagnostics, especially in an anatomical pathology laboratory. ISH is a technique that can detect the targeted DNA or RNA sequences in tissue sections from frozen or fixed materials with labeled DNA or RNA probes. Radioactive and non-radioactive probes are the two major probes that can be used to label the targeted nucleic acids. Areas covered: Two decades after the Human Genome Project, ISH has not only simply been applied to identify the chromosomal location of a human gene but has also been extensively applied to gene expressions studies and utilized for clinical diagnosis, especially for the determination of biomarkers for breast and ovarian cancers–human epidermal growth factor receptor 2. Duchenne muscular dystrophy, Cri-du-chat syndrome, Angelman syndrome, PraderWilli syndrome, cystic fibrosis, and trisomy are diseases that can also be detected by ISH. In this review, the basic principles, historical development, advantages and disadvantages, enhancement in reporting molecules and probes, advancement in detection methods, in situ PCR, clinical applications and novel applications of ISH will be discussed. Expert opinion: With the advancement in ISH technologies and appropriate training, diagnosis can be improved in Anatomical Pathology.
KW - Cancer diagnosis
KW - in situ hybridization
KW - in situ PCR
KW - molecular diagnosis
UR - http://www.scopus.com/inward/record.url?scp=85119985472&partnerID=8YFLogxK
U2 - 10.1080/14737159.2022.2007076
DO - 10.1080/14737159.2022.2007076
M3 - Journal article
C2 - 34779317
AN - SCOPUS:85119985472
SN - 1473-7159
VL - 22
SP - 5
EP - 18
JO - Expert Review of Molecular Diagnostics
JF - Expert Review of Molecular Diagnostics
IS - 1
ER -