TGF-β1 promotes motility and invasiveness of glioma cells through activation of ADAM17

Yong Lu, Feng Jiang, Xuguang Zheng, Mark Katakowski, Benjamin Buller, Shing Shun Tony To, Michael Chopp

Research output: Journal article publicationJournal articleAcademic researchpeer-review

41 Citations (Scopus)

Abstract

The transforming growth factor β1 (TGF-β1) belongs to a family of structurally related polypeptide factors. TGF-beta plays an important role in the pathobiology of invasion of malignant gliomas. The objective of the present study was to investigate the impact of TNF-α converting enzyme (TACE/ADAM17) signaling on the process of TGF-β1-stimulated migration and invasion of T98G glioma cells. We found that TGF-β1 increased migration and invasiveness in glioma cells. Addition of the TGF-β1 receptor inhibitor, SB431542, reduced the TGF-β1-stimulated migration and invasiveness of glioma cells. In addition, TGF-β1-induced migration and invasiveness were also blocked by exposure to an ADAM17 inhibitor, TAPI-2. Furthermore, ADAM17 mRNA and protein expression were up-regulated by TGF-β1. Treatment with SB431542 and TAPI-2 blocked TGF-β1-induced ADAM17 protein expression. In summary, these results indicate that TGF-β1 promotes cell migration and invasiveness of glioma cells through stimulation of ADAM17.
Original languageEnglish
Pages (from-to)1329-1335
Number of pages7
JournalOncology Reports
Volume25
Issue number5
DOIs
Publication statusPublished - 1 May 2011

Keywords

  • ADAM17
  • Glioma
  • Invasion
  • Migration
  • Transforming growth factor β1

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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