Telomerase-associated apoptotic events by mushroom Ganoderma lucidum on premalignant human urothelial cells

Wai Man Yuen, Mayur Danny I Gohel, Doris Wai Ting Au

Research output: Journal article publicationJournal articleAcademic researchpeer-review

24 Citations (Scopus)

Abstract

The chemopreventive effects of Ganoderma lucidum was tested, using a tumorigenic transformable human urothelial cell (HUC-PC) model. These in vitro data show that G. lucidum can inhibit the viability and growth of HUC-PC. This could be explained by a concomitant induction of apoptosis and inhibition of telomerase activity. Significant exteriorization of phosphatidylserine was detected by Annexin-V on cell surface, and the cells subsequently lost membrane integrity for uptake of 7-amino-actinomycin D dye. Additionally, the levels of hydrogen peroxide and 8-hydroxy-2′-deoxyguanosine (8-OHdG) production of the apoptotic cells were significantly increased. The induction of apoptosis and suppression of telomerase activity help to explain the anti-HUC-PC growth properties; however, the induction of oxidative stress requires further study. This study strongly suggests that G. lucidum is a potential source of chemopreventive agents for bladder cancer based on its effectiveness in controlling the premalignant urothelial cell growth and carcinogen-induced transformation.
Original languageEnglish
Pages (from-to)109-119
Number of pages11
JournalNutrition and Cancer
Volume60
Issue number1
DOIs
Publication statusPublished - 1 Jan 2008

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Oncology
  • Food Science

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