TALEN-Mediated mutagenesis and genome editing

Chun Hang Ma; Yi Chen; Patrick R. Blackburn; Stephen C. Ekker

doi:10.1007/978-1-4939-3771-4_2

TALEN-Mediated mutagenesis and genome editing

Chun Hang Ma, Yi Chen, Patrick R. Blackburn, Stephen C. Ekker

Research output: Chapter in book / Conference proceeding › Chapter in an edited book (as author) › Academic research › peer-review

21 Citations (Scopus)

Abstract

Transcription activator-like effectors (TALEs) are important genomic tools with customizable DNA-binding motifsfor locus-specific modifications. In particular, TALE nucleases or TALENs have been successfully used in the zebrafish model system to introduce targeted mutations via repair of double-stranded breaks (DSBs) either through nonhomologous end joining (NHEJ) or by homology-directed repair (HDR) and homology-independent repair in the presence of a donor template. Compared with other customizable nucleases, TALENs offer high binding specificity and fewer sequence constraints in targeting the genome, with comparable mutagenic activity. Here, we describe a detailed in silico design tool for zebrafish genome editing for TALENs and CRISPR/Cas9 custom restriction enzymes using Mojo Hand 2.0 software.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	17-30
Number of pages	14
DOIs	https://doi.org/10.1007/978-1-4939-3771-4_2
Publication status	Published - 1 Jan 2016

Publication series

Name	Methods in Molecular Biology
Volume	1451
ISSN (Print)	1064-3745

Keywords

Customized nucleases
FusX
Genome editing
Golden Gate
TALEN
Zebrafish

ASJC Scopus subject areas

Molecular Biology
Genetics

More information

10.1007/978-1-4939-3771-4_2

Cite this

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TALEN-Mediated mutagenesis and genome editing

Abstract

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Keywords

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