@inbook{1aa7c3df62d647928eb5395e0564f967,
title = "TALEN-Mediated mutagenesis and genome editing",
abstract = "Transcription activator-like effectors (TALEs) are important genomic tools with customizable DNA-binding motifsfor locus-specific modifications. In particular, TALE nucleases or TALENs have been successfully used in the zebrafish model system to introduce targeted mutations via repair of double-stranded breaks (DSBs) either through nonhomologous end joining (NHEJ) or by homology-directed repair (HDR) and homology-independent repair in the presence of a donor template. Compared with other customizable nucleases, TALENs offer high binding specificity and fewer sequence constraints in targeting the genome, with comparable mutagenic activity. Here, we describe a detailed in silico design tool for zebrafish genome editing for TALENs and CRISPR/Cas9 custom restriction enzymes using Mojo Hand 2.0 software.",
keywords = "Customized nucleases, FusX, Genome editing, Golden Gate, TALEN, Zebrafish",
author = "Ma, \{Chun Hang\} and Yi Chen and Blackburn, \{Patrick R.\} and Ekker, \{Stephen C.\}",
year = "2016",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-3771-4\_2",
language = "English",
series = "Methods in Molecular Biology",
pages = "17--30",
booktitle = "Methods in Molecular Biology",
}