TY - JOUR
T1 - Synthesis of fluorescent nanoprobe with simultaneous response to intracellular pH and Zn2+ for tumor cell distinguishment
AU - Wang, Qin
AU - Shi, Yupeng
AU - Chen, Wandi
AU - Yang, Mo
AU - Yi, Changqing
N1 - Funding Information:
This work was partially supported by the National Scientific Foundation of China (81901808) and the Guangdong Natural Science Foundation (S2017A030313076, 2020A1515010661).
Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, AT part of Springer Nature.
PY - 2021/1/2
Y1 - 2021/1/2
N2 - A novel dual-functional nanoprobe was designed and synthesized by facile assembly of quinoline derivative (PEIQ) and meso-tetra (4-carboxyphenyl) porphine (TCPP) via electrostatic interaction for simultaneous sensing of fluorescence of Zn2+ and pH. Under the single-wavelength excitation at 400 nm, this nanoprobe not only exhibits “OFF-ON” green fluorescence at 512 nm by specific PEIQ-Zn2+ chelation, but also presents red fluorescence enhancement at 654 nm by H+-triggered TCPP release. The nanoprobe demonstrated excellent sensing performance with a good linear range (Zn2+, 1–40 μM; pH, 5.0–8.0), low detection limit (Zn2+, 0.88 μM), and simultaneous response towards Zn2+ and pH in pure aqueous solution within 2 min. More importantly, this dual-functional nanoprobe demonstrates the capability of discerning cancerous cells from normal cells, as evidenced by the fact that cancerous HepG2 cells in tumor microenvironment exhibit substantially higher red fluorescence and significantly lower green fluorescence than normal HL-7702 cells. The simultaneous, real-time fluorescence imaging of multiple analytes in a living system could be significant for cell analysis and tracking, cancer diagnosis, and even fluorescence-guided surgery of tumors. Graphical abstract: [Figure not available: see fulltext.].
AB - A novel dual-functional nanoprobe was designed and synthesized by facile assembly of quinoline derivative (PEIQ) and meso-tetra (4-carboxyphenyl) porphine (TCPP) via electrostatic interaction for simultaneous sensing of fluorescence of Zn2+ and pH. Under the single-wavelength excitation at 400 nm, this nanoprobe not only exhibits “OFF-ON” green fluorescence at 512 nm by specific PEIQ-Zn2+ chelation, but also presents red fluorescence enhancement at 654 nm by H+-triggered TCPP release. The nanoprobe demonstrated excellent sensing performance with a good linear range (Zn2+, 1–40 μM; pH, 5.0–8.0), low detection limit (Zn2+, 0.88 μM), and simultaneous response towards Zn2+ and pH in pure aqueous solution within 2 min. More importantly, this dual-functional nanoprobe demonstrates the capability of discerning cancerous cells from normal cells, as evidenced by the fact that cancerous HepG2 cells in tumor microenvironment exhibit substantially higher red fluorescence and significantly lower green fluorescence than normal HL-7702 cells. The simultaneous, real-time fluorescence imaging of multiple analytes in a living system could be significant for cell analysis and tracking, cancer diagnosis, and even fluorescence-guided surgery of tumors. Graphical abstract: [Figure not available: see fulltext.].
KW - Fluorescence nanoprobe
KW - Hepatoma
KW - HepG2 cells
KW - HL-7702 cells
KW - Ratiometric fluorescence
KW - Supramolecular nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85098481447&partnerID=8YFLogxK
U2 - 10.1007/s00604-020-04682-0
DO - 10.1007/s00604-020-04682-0
M3 - Journal article
C2 - 33389210
AN - SCOPUS:85098481447
SN - 0026-3672
VL - 188
SP - 9
EP - 9
JO - Microchimica Acta
JF - Microchimica Acta
IS - 1
ER -