Synthesis of a precursor of bioactive pentapeptide OGP-(10-14) and the fragment of enkephalin catalyzed by MCM-22 immobilized or free proteases in organic solvents

Ping Liu, Yun Hua Ye, Gui Ling Tian, Kin Sing Lee, Man Sau Wong, Wai Hung Lo

Research output: Journal article publicationJournal articleAcademic researchpeer-review

7 Citations (Scopus)

Abstract

Full enzymatic synthesis of a precursor of osteogenic growth peptide fragment (10-14), Z-Tyr-Gly-Phe-Gly-Gly-OEt, was accomplished using immobilized proteases on molecular sieve MCM-22 by adsorption as catalyst via 3+2 synthetic route for the first rime. The reuse time of immobilized enzyme was dependent on concrete reaction system. Compared with free enzyme, the reaction rate catalyzed by immobilized enzyme was remarkably enhanced and its synthetic yield was also increased in most cases. The effects of water content and the added amount of Et3N on synthesis of fragments of bioactive peptides including OGP-(10-14) and enkephalin by immobilized enzymes were different from free enzyme.
Original languageEnglish
Pages (from-to)726-732
Number of pages7
JournalSynthesis
Issue number6
Publication statusPublished - 20 May 2002

Keywords

  • Enzymatic peptide synthesis
  • Immobilized enzyme
  • MCM-22
  • OGP-(10-14)
  • Organic solvents

ASJC Scopus subject areas

  • Catalysis
  • Organic Chemistry

Cite this