Surface-plasmon microscopy with a two-piece solid immersion lens: Bright and dark fields

Jing Zhang, Mark C. Pitter, Shugang Liu, Chungwah See, Michael Geoffrey Somekh

Research output: Journal article publicationJournal articleAcademic researchpeer-review

23 Citations (Scopus)


We report bright-field and dark-field surface-plasmon imaging using a modified solid immersion lens and a commercial objective of moderate NA in the epi configuration. The contrast and resolution are extremely good, giving well-resolved images of protein monolayers both in air and in water. We also describe a two-part solid immersion lens that allows the sample to be moved without degrading the image quality in any observable way. The merits of the two-part lens are discussed and compared to commercially available microscope objectives. Finally, we introduce a simple Green's function model that illustrates the key features of both bright-field and dark-field surface-plasmon imaging.
Original languageEnglish
Pages (from-to)7977-7986
Number of pages10
JournalApplied Optics
Issue number31
Publication statusPublished - 1 Nov 2006
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics


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