Sulfation and enhanced antioxidant capacity of an exopolysaccharide produced by the medicinal fungus Cordyceps sinensis

Jing Kun Yan, Wen Qiang Wang, Hai Le Ma, Jianyong Wu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

26 Citations (Scopus)


EPS-1 was an exopolysaccharide produced by the medicinal fungus Cordyceps sinensis (Cs-HK1). In the present study, EPS-1 was sulfated with chlorosulfonic acid (CSA)-pyridine (Pyr) at different volume ratios, yielding four sulfated derivatives, SEPS-1A, B, C and D, with different degrees of substitution (DS: 0.25-1.38) and molecular weights (17.1-4.1 kDa). The sulfation of EPS-1 occurred most frequently at the C-6 hydroxyl groups due to their higher reactivity. In aqueous solution, the native EPS-1 formed random coils or aggregated networks, but the sulfated derivatives formed single helices. The antioxidant activities of the sulfated EPS-1 derivatives for scavenging hydroxyl radicals (·OH) and 2,2-Azinobis-3-ehtylbenzothiazolin-6-sulfonic acid radicals (ABTS·+) were significantly increased with increasing DS and decreasing molecular weight (MW). Sulfation has thus been shown to be an effective and favorable strategy for improving the physico-chemical properties and bioactivities of fungal polysaccharides.
Original languageEnglish
Pages (from-to)167-177
Number of pages11
Issue number1
Publication statusPublished - 1 Jan 2013


  • Antioxidant activity
  • Cordyceps sinensis
  • Exopolysaccharide
  • Molecular weight
  • Sulfation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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