Substrate-related factors affecting cellulosome-induced hydrolysis for lignocellulose valorization

Ying Wang, Ling Leng, Md Khairul Islam, Fanghua Liu, Carol Sze Ki Lin, Shao Yuan Leu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

16 Citations (Scopus)


Cellulosomes are an extracellular supramolecular multienzyme complex that can efficiently degrade cellulose and hemicelluloses in plant cell walls. The structural and unique subunit arrangement of cellulosomes can promote its adhesion to the insoluble substrates, thus providing individual microbial cells with a direct competence in the utilization of cellulosic biomass. Significant progress has been achieved in revealing the structures and functions of cellulosomes, but a knowledge gap still exists in understanding the interaction between cellulosome and lignocellulosic substrate for those derived from biorefinery pretreatment of agricultural crops. The cellulosomic saccharification of lignocellulose is affected by various substrate-related physical and chemical factors, including native (untreated) wood lignin content, the extent of lignin and xylan removal by pretreatment, lignin structure, substrate size, and of course substrate pore surface area or substrate accessibility to cellulose. Herein, we summarize the cellulosome structure, substrate-related factors, and regulatory mechanisms in the host cells. We discuss the latest advances in specific strategies of cellulosome-induced hydrolysis, which can function in the reaction kinetics and the overall progress of biorefineries based on lignocellulosic feedstocks.

Original languageEnglish
Article number3354
JournalInternational Journal of Molecular Sciences
Issue number13
Publication statusPublished - 1 Jul 2019


  • Bioproducts
  • Cellulosome
  • Enzymatic hydrolysis
  • Lignocellulose
  • Reaction kinetics
  • Substrate-related factors

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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