Study of high-throughput cell electrofusion in a microelectrode-array chip

Yi Cao, Jun Yang, Zheng Qin Yin, Hong Yan Luo, Mo Yang, Ning Hu, Jing Yang, Dan Qun Huo, Chang Jun Hou, Zhi Zhong Jiang, Rui Qiang Zhang, Rong Xu, Xiao Lin Zheng

Research output: Journal article publicationJournal articleAcademic researchpeer-review

36 Citations (Scopus)

Abstract

A microfabricated high-throughput cell electrofusion chip with 1,368 pairs of high aspect ratio silicon microelectrodes is presented. These microelectrodes, which were distributed in six individual microscale cell-fusion chambers, were covered with titanium and gold thin film to improve their electric conductivity as well as surface hydrophobility. Six chambers having different electrode distances make the chip highly suitable for fusing cells with different sizes. A microfluidic platform was set up for flowing control, cell manipulation and also experimental observation. Cells for electrofusion were first aligned at the prearranged locations by the dielectrophoretic force between two counter-electrodes, which benefits the traverse of electric pulse through the cell-cell contacting point for electroporation. Several on-chip cell electrofusion experiments have been carried out on different kinds of animal cells and plant protoplasts. Compared with conventional electrofusion methods, higher fusion efficiency was achieved on this device for precisely forming micropores on the proximate membranes of two contacting cells, and high throughput was also obtained due to the use of a large number of microelectrodes for cell manipulation and fusion. Moreover, a much lower power supply was required for the shorter distance between two counter-electrodes.
Original languageEnglish
Pages (from-to)669-675
Number of pages7
JournalMicrofluidics and Nanofluidics
Volume5
Issue number5
DOIs
Publication statusPublished - 4 Apr 2008

Keywords

  • Cell fusion
  • Electrofusion
  • Microelectrode array
  • Microfluidic

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Materials Chemistry

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