TY - JOUR
T1 - Structure, anti-fatigue activity and regulation on gut microflora in vivo of ethanol-fractional polysaccharides from Dendrobium officinale
AU - Cai, Ming
AU - Zhu, Hua
AU - Xu, Lei
AU - Wang, Jian
AU - Xu, Jing
AU - Li, Zhenhao
AU - Yang, Kai
AU - Wu, Jianyong
AU - Sun, Peilong
N1 - Funding Information:
This work was supported financially by the Key Research and Development Projects of Zhejiang ( 2019C02100 ).
Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/4/15
Y1 - 2023/4/15
N2 - This study was to investigate the antifatigue, prebiotic effects and their relationships to the structure properties of three ethanol precipitated polysaccharides from Dendrobium officinale (EPDO), as EPDO-40, EPDO-60 and EPDO-80. EPDOs with anti-fatigue activity were screened out by forced swimming test, and blood lactic acid (BLA), blood urea nitrogen (BUN), superoxide dismutase (SOD), liver glycogen, muscle glycogen, and intestinal microflora were investigated. Results showed that purified EPDO-60, 277.3 kDa, with a backbone consisted of 4-Manp and 4-Glcp. EPDO-60 had the best anti-fatigue activity, because it could significantly prolong the forced swimming time, as well as down-regulating the levels of BLA and BUN, increasing SOD. Proportions of Bacteroidetes and Firmicutes and abundance of Lactobacillus and Bifidobacterium in gut microflora increased after treated with EPDO-60. Accordingly, EPDO-60 could affect the community structure of gut microflora, leading to promote the balance of oxidation and antioxidation, and accelerated the fatigue metabolism in vivo.
AB - This study was to investigate the antifatigue, prebiotic effects and their relationships to the structure properties of three ethanol precipitated polysaccharides from Dendrobium officinale (EPDO), as EPDO-40, EPDO-60 and EPDO-80. EPDOs with anti-fatigue activity were screened out by forced swimming test, and blood lactic acid (BLA), blood urea nitrogen (BUN), superoxide dismutase (SOD), liver glycogen, muscle glycogen, and intestinal microflora were investigated. Results showed that purified EPDO-60, 277.3 kDa, with a backbone consisted of 4-Manp and 4-Glcp. EPDO-60 had the best anti-fatigue activity, because it could significantly prolong the forced swimming time, as well as down-regulating the levels of BLA and BUN, increasing SOD. Proportions of Bacteroidetes and Firmicutes and abundance of Lactobacillus and Bifidobacterium in gut microflora increased after treated with EPDO-60. Accordingly, EPDO-60 could affect the community structure of gut microflora, leading to promote the balance of oxidation and antioxidation, and accelerated the fatigue metabolism in vivo.
KW - Anti-fatigue
KW - Dendrobium officinale
KW - Gut microflora
KW - Polysaccharides
UR - http://www.scopus.com/inward/record.url?scp=85147983856&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2023.123572
DO - 10.1016/j.ijbiomac.2023.123572
M3 - Journal article
C2 - 36754265
AN - SCOPUS:85147983856
SN - 0141-8130
VL - 234
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
M1 - 123572
ER -