Structure and properties of a (1→3)-β-d-glucan from ultrasound-degraded exopolysaccharides of a medicinal fungus

Xia Chen, Ka Chai Siu, Yi Ching Cheung, Jianyong Wu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

27 Citations (Scopus)

Abstract

A high molecular weight (MW) exopolysaccaride (EPS) fraction EPS1 was isolated from the fermentation broth of a medicinal fungus Cordyceps sinensis Cs-HK1 and partially degraded by high-intensity ultrasound (US) into a lower MW fraction EPS1U. EPS1U exhibited a single, symmetric peak on size exclusion chromatography with an average MW of 730 kDa by light scattering analysis. It had a much lower intrinsic viscosity (1.7 versus 15.6 dL/g) but a much higher solubility in water (77.5 versus 5.1 g/L) than EPS1. Based on methylation analysis and NMR spectrometry, the structure of EPS1U was deduced as a (1→3)-β-d-glucan with glucose side chains attached to O-6 position at the branching points. EPS1U showed a high moisture absorption capability comparable to chitosan and urea, suggesting its potential as a moisturizing agent for food and cosmeceutical application. This is the first report on a high MW (1→3)-β-d-glucan isolated from EPS produced by Cordyceps sinensis.
Original languageEnglish
Pages (from-to)270-275
Number of pages6
JournalCarbohydrate Polymers
Volume106
Issue number1
DOIs
Publication statusPublished - 15 Jun 2014

Keywords

  • (1→3)-β-d-glucan
  • Cordyceps sinensis
  • Exopolysaccharide
  • Moisture absorption
  • Mycelial fermentation
  • Ultrasonic degradation

ASJC Scopus subject areas

  • Organic Chemistry
  • Materials Chemistry
  • Polymers and Plastics
  • Medicine(all)

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