Stochastic transfer function: Application to fluorescence microscopy

Michael Geoffrey Somekh, Ken Hsu, Mark C. Pitter

Research output: Journal article publicationJournal articleAcademic researchpeer-review

3 Citations (Scopus)

Abstract

We develop the concept of the stochastic transfer function (STF) and its application to high-resolution fluorescencemicroscopy. The STF is directly related to the conventional optical transfer functions but incorporates a probability density function at each spatial frequency. The mean of the STF yields the conventional transfer function; the variance of the STF gives a measure of the noise associated at each spatial frequency. The STF thus provides a more complete picture of the microscope performance in comparison with conventional transfer functions. Here we present the STF for a wide-field fluorescent microscope using both Monte Carlo simulation and probability theory. © 2009 Optical Society of America.
Original languageEnglish
Pages (from-to)1622-1629
Number of pages8
JournalJournal of the Optical Society of America A: Optics and Image Science, and Vision
Volume26
Issue number7
DOIs
Publication statusPublished - 1 Jul 2009
Externally publishedYes

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Computer Vision and Pattern Recognition

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