Abstract
We develop the concept of the stochastic transfer function (STF) and its application to high-resolution fluorescencemicroscopy. The STF is directly related to the conventional optical transfer functions but incorporates a probability density function at each spatial frequency. The mean of the STF yields the conventional transfer function; the variance of the STF gives a measure of the noise associated at each spatial frequency. The STF thus provides a more complete picture of the microscope performance in comparison with conventional transfer functions. Here we present the STF for a wide-field fluorescent microscope using both Monte Carlo simulation and probability theory. © 2009 Optical Society of America.
Original language | English |
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Pages (from-to) | 1622-1629 |
Number of pages | 8 |
Journal | Journal of the Optical Society of America A: Optics and Image Science, and Vision |
Volume | 26 |
Issue number | 7 |
DOIs | |
Publication status | Published - 1 Jul 2009 |
Externally published | Yes |
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Computer Vision and Pattern Recognition