TY - JOUR
T1 - Stimulated Angiogenesis for Fracture Healing Augmented by Low-Magnitude, High-Frequency Vibration in A Rat Model-Evaluation of Pulsed-Wave Doppler, 3-D Power Doppler Ultrasonography and Micro-CT Microangiography
AU - Cheung, Wing Hoi
AU - Sun, Ming Hui
AU - Zheng, Yongping
AU - Chu, Winnie Chiu Wing
AU - Leung, Andraay Hon Chi
AU - Qin, Ling
AU - Wei, Fang Yuan
AU - Leung, Kwok Sui
PY - 2012/12/1
Y1 - 2012/12/1
N2 - This study aimed to investigate the mechanism of low-magnitude high-frequency vibration (LMHFV) treatment on angiogenesis and blood flow for enhancement of fracture healing. Nine-month-old ovariectomized (OVX) and sham-operated (Sham) rats received closed fractures creation at the femora and were randomized into LMHFV treatment (Sham-V, OVX-V) or control (Sham-C, OVX-C) groups. Pulsed-wave Doppler indicated an increase in blood flow velocity of the femoral artery at weeks 2 (OVX pair: p = 0.030) and 4 (OVX pair: p = 0.012; Sham pair: p = 0.020) post-treatment. Significantly enhanced vascular volume (VV) at the fracture site in the vibration groups was demonstrated by 3-D high-frequency power Doppler at week 2 (Sham pair: p = 0.021) and micro-computed tomography (microCT) microangiography at weeks 2 (OVX pair: p = 0.009) and 4 (OVX pair: p = 0.034), which echoed the osteogenesis findings by radiographic and microCT analysis. VV in the OVX groups was inferior to the Sham groups. However, OVX-V showed higher percentages of angiogenic enhancement than Sham-V. Despite impaired neo-angiogenesis in osteoporotic fractures, LMHFV could increase blood flow and angiogenesis in both normal and osteoporotic fractures, thus enhancing fracture healing.
AB - This study aimed to investigate the mechanism of low-magnitude high-frequency vibration (LMHFV) treatment on angiogenesis and blood flow for enhancement of fracture healing. Nine-month-old ovariectomized (OVX) and sham-operated (Sham) rats received closed fractures creation at the femora and were randomized into LMHFV treatment (Sham-V, OVX-V) or control (Sham-C, OVX-C) groups. Pulsed-wave Doppler indicated an increase in blood flow velocity of the femoral artery at weeks 2 (OVX pair: p = 0.030) and 4 (OVX pair: p = 0.012; Sham pair: p = 0.020) post-treatment. Significantly enhanced vascular volume (VV) at the fracture site in the vibration groups was demonstrated by 3-D high-frequency power Doppler at week 2 (Sham pair: p = 0.021) and micro-computed tomography (microCT) microangiography at weeks 2 (OVX pair: p = 0.009) and 4 (OVX pair: p = 0.034), which echoed the osteogenesis findings by radiographic and microCT analysis. VV in the OVX groups was inferior to the Sham groups. However, OVX-V showed higher percentages of angiogenic enhancement than Sham-V. Despite impaired neo-angiogenesis in osteoporotic fractures, LMHFV could increase blood flow and angiogenesis in both normal and osteoporotic fractures, thus enhancing fracture healing.
KW - Angiogenesis
KW - Doppler ultrasonography
KW - Fracture healing
KW - Low-magnitude high-frequency vibration
KW - MicroCT microangiography
UR - http://www.scopus.com/inward/record.url?scp=84870299028&partnerID=8YFLogxK
U2 - 10.1016/j.ultrasmedbio.2012.07.025
DO - 10.1016/j.ultrasmedbio.2012.07.025
M3 - Journal article
C2 - 23062367
SN - 0301-5629
VL - 38
SP - 2120
EP - 2129
JO - Ultrasound in Medicine and Biology
JF - Ultrasound in Medicine and Biology
IS - 12
ER -