Stimulated Angiogenesis for Fracture Healing Augmented by Low-Magnitude, High-Frequency Vibration in A Rat Model-Evaluation of Pulsed-Wave Doppler, 3-D Power Doppler Ultrasonography and Micro-CT Microangiography

Wing Hoi Cheung, Ming Hui Sun, Yongping Zheng, Winnie Chiu Wing Chu, Andraay Hon Chi Leung, Ling Qin, Fang Yuan Wei, Kwok Sui Leung

Research output: Journal article publicationJournal articleAcademic researchpeer-review

39 Citations (Scopus)

Abstract

This study aimed to investigate the mechanism of low-magnitude high-frequency vibration (LMHFV) treatment on angiogenesis and blood flow for enhancement of fracture healing. Nine-month-old ovariectomized (OVX) and sham-operated (Sham) rats received closed fractures creation at the femora and were randomized into LMHFV treatment (Sham-V, OVX-V) or control (Sham-C, OVX-C) groups. Pulsed-wave Doppler indicated an increase in blood flow velocity of the femoral artery at weeks 2 (OVX pair: p = 0.030) and 4 (OVX pair: p = 0.012; Sham pair: p = 0.020) post-treatment. Significantly enhanced vascular volume (VV) at the fracture site in the vibration groups was demonstrated by 3-D high-frequency power Doppler at week 2 (Sham pair: p = 0.021) and micro-computed tomography (microCT) microangiography at weeks 2 (OVX pair: p = 0.009) and 4 (OVX pair: p = 0.034), which echoed the osteogenesis findings by radiographic and microCT analysis. VV in the OVX groups was inferior to the Sham groups. However, OVX-V showed higher percentages of angiogenic enhancement than Sham-V. Despite impaired neo-angiogenesis in osteoporotic fractures, LMHFV could increase blood flow and angiogenesis in both normal and osteoporotic fractures, thus enhancing fracture healing.
Original languageEnglish
Pages (from-to)2120-2129
Number of pages10
JournalUltrasound in Medicine and Biology
Volume38
Issue number12
DOIs
Publication statusPublished - 1 Dec 2012

Keywords

  • Angiogenesis
  • Doppler ultrasonography
  • Fracture healing
  • Low-magnitude high-frequency vibration
  • MicroCT microangiography

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Biophysics
  • Acoustics and Ultrasonics

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