Spread of Carbapenemase-producing Enterobacteria in a Southwest Hospital in China

Sheng Chen, Wei Feng, Jianhong Chen, Wei Liao, Nianhai He, Qian Wang, Fengjun Sun, Peiyuan Xia

Research output: Journal article publicationJournal articleAcademic researchpeer-review

11 Citations (Scopus)

Abstract

Background: The rapid emergence and dissemination of carbapenem resistance in Enterobacteriaceae complicates the treatment of infections caused by these organisms.Methods: We collected clinical isolates with meropenem inhibition zones of ≤ 22 mm from January 1, 2009, through December 31, 2010. We attempted to amplify the NDM-1 gene from these isolates and conducted the modified Hodge test (MHT). The minimal inhibitory concentration (MIC) of the MHT-positive strains was determined by the agar disk dilution method. The carbapenemase-encoding resistance genes of these strains were examined using polymerase chain reaction (PCR) analysis and a sequencing strategy to characterize these enzymes. The clonal relationship among isolates was analyzed by pulsed-field gel electrophoresis (PFGE). Results: Among the 158 Enterobacteriaceae isolates that were collected, there were no NDM-1-positive strains and 26 MHT-positive strains. Among the latter, 18 strains were IMP-4-positive, and 1 was KPC-2-positive. In addition, 15 of the IMP-4-positive Klebsiella pneumoniae strains belonged to 4 PFGE genotypes, with 8 strains having the same genotype.Conclusion: These results suggest that nosocomial infections are one of the main reasons for the spread of these resistant strains.
Original languageEnglish
Article number42
JournalAnnals of Clinical Microbiology and Antimicrobials
Volume13
Issue number1
DOIs
Publication statusPublished - 12 Aug 2014
Externally publishedYes

Keywords

  • Carbapenemase
  • Emergence
  • Enterobacteria
  • Resistance
  • Spread

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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