Sphinganine potentiation of dimethyl sulfoxide-induced granulocytic differentiation, increase of alkaline phosphatase activity and decrease of protein kinase C activity in a human leukemia cell line (HL-60)

Yat Ming Yung, Te Fang Hsiao, Lillian Li Lin Wei, Eric Ka Wai Hui

Research output: Journal article publicationJournal articleAcademic researchpeer-review

11 Citations (Scopus)


The differentiation of HL-60 promyelocytic cells toward mature granulocytic cells induced by dimethyl sulfoxide (DMSO) was accompanied by a quantitative similar increase in alkaline phosphatase (ALP) activity and decrease in protein kinase C (PKC) activity. The combination of DMSO and sphinganine (SP), a potent inhibitor of PKC, increased in parallel the percentage of mature cells and the ALP activity. The enhancement of DMSO-induced differentiation and the potentiation of the decrease of PKC activity by SP also seemed to correlate with each other. Our results indicate that both ALP and PKC may play a role in the DMSO-induced granulocytic differentiation. Inc.
Original languageEnglish
Pages (from-to)888-896
Number of pages9
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 1 Jan 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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