Sp family members stimulate transcription of the Hex gene via interactions with GC boxes

Eri Kikkawa, Makiyo Hinata, Wee-Keong Vincent Keng, Zaw Myint, Ayuko Sato, Kazuya Yamada, Takashi Tanaka, Tamio Noguchi

Research output: Journal article publicationJournal articleAcademic researchpeer-review

10 Citations (Scopus)

Abstract

The 5′-flanking region of the mouse Hex gene was examined in order to identify transcription factors regulating its expression in hepatocytes and haematopoietic cells. We have identified two further GC boxes (GC boxes 3 and 4 at nucleotide positions −149 to −140 and −79 to −70, respectively), i.e. in addition to the two previously determined ones (GC boxes 1 and 2 at nucleotide positions −197 to −188 and −176 to −167, respectively). Luciferase reporter assays revealed that all four GC boxes are transcriptionally active in both MH1C1rat hepatoma and K562 human chronic myelogenous leukemia cells. Electrophoretic mobility shift assays with specific competitors and antibodies showed that members of the Sp family, namely Sp1 and Sp3, bind to these GC boxes. Overexpression of Sp1 and Sp3 in Drosophila SL2 cells stimulated transcription of the Hex gene through interactions with GC boxes 1 to 4, Sp1 being a more potent activator than Sp3. Thus, we conclude that Sp1 and Sp3 stimulate transcription of the Hex gene in both MH1C1and K562 cells.
Original languageEnglish
Pages (from-to)885-891
Number of pages7
JournalJournal of Biochemistry
Volume130
Issue number6
DOIs
Publication statusPublished - 1 Dec 2001
Externally publishedYes

Keywords

  • Differentiation
  • Haematopoietic cell
  • Hepatocyte
  • Homeobox gene
  • Transcription factor

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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