Soft hydrogel promotes dorsal root ganglion by upregulating gene expression of Ntn4 and Unc5B

Liling Zhang, Qi Han, Shiyu Chen, Di Suo, Luzhong Zhang, Guicai Li, Xin Zhao, Yumin Yang

Research output: Journal article publicationJournal articleAcademic researchpeer-review

1 Citation (Scopus)

Abstract

Mechanical property is an important factor of cellular microenvironment for neural tissue regeneration. In this study, polyacrylamide (PAM) hydrogels with systematically varying elastic modulus were prepared using in situ radical polymerization. We found that the hydrogel was biocompatible, and the length of dorsal root ganglion (DRG)'s axon and cell density were optimal on the hydrogels with elastic modulus of 5.1 kPa (among hydrogels with elastic modulus between 3.6 kPa and 16.5 kPa). These DRGs also exhibited highest gene and protein expression of proliferation marker Epha4, Ntn4, Sema3D and differentiation marker Unc5B. Our study revealed the mechanism of how material stiffness affects DRG proliferation and differentiation. It will also provide theoretical basis and evidence for the design and development of nerve graft with better repair performance.

Original languageEnglish
Article number111503
JournalColloids and Surfaces B: Biointerfaces
Volume199
DOIs
Publication statusPublished - Mar 2021

Keywords

  • Dorsal root ganglion
  • Gene and protein expression
  • Polyacrylamide
  • Stiffness

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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