Soft hydrogel promotes dorsal root ganglion by upregulating gene expression of Ntn4 and Unc5B

Liling Zhang; Qi Han; Shiyu Chen; Di Suo; Luzhong Zhang; Guicai Li; Xin Zhao; Yumin Yang

doi:10.1016/j.colsurfb.2020.111503

Soft hydrogel promotes dorsal root ganglion by upregulating gene expression of Ntn4 and Unc5B

Liling Zhang, Qi Han, Shiyu Chen, Di Suo, Luzhong Zhang, Guicai Li, Xin Zhao, Yumin Yang

Research output: Journal article publication › Journal article › Academic research › peer-review

8 Citations (Scopus)

Abstract

Mechanical property is an important factor of cellular microenvironment for neural tissue regeneration. In this study, polyacrylamide (PAM) hydrogels with systematically varying elastic modulus were prepared using in situ radical polymerization. We found that the hydrogel was biocompatible, and the length of dorsal root ganglion (DRG)'s axon and cell density were optimal on the hydrogels with elastic modulus of 5.1 kPa (among hydrogels with elastic modulus between 3.6 kPa and 16.5 kPa). These DRGs also exhibited highest gene and protein expression of proliferation marker Epha4, Ntn4, Sema3D and differentiation marker Unc5B. Our study revealed the mechanism of how material stiffness affects DRG proliferation and differentiation. It will also provide theoretical basis and evidence for the design and development of nerve graft with better repair performance.

Original language	English
Article number	111503
Journal	Colloids and Surfaces B: Biointerfaces
Volume	199
DOIs	https://doi.org/10.1016/j.colsurfb.2020.111503
Publication status	Published - Mar 2021

Keywords

Dorsal root ganglion
Gene and protein expression
Polyacrylamide
Stiffness

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

More information

10.1016/j.colsurfb.2020.111503

http://hdl.handle.net/10397/92828

Cite this

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title = "Soft hydrogel promotes dorsal root ganglion by upregulating gene expression of Ntn4 and Unc5B",

abstract = "Mechanical property is an important factor of cellular microenvironment for neural tissue regeneration. In this study, polyacrylamide (PAM) hydrogels with systematically varying elastic modulus were prepared using in situ radical polymerization. We found that the hydrogel was biocompatible, and the length of dorsal root ganglion (DRG)'s axon and cell density were optimal on the hydrogels with elastic modulus of 5.1 kPa (among hydrogels with elastic modulus between 3.6 kPa and 16.5 kPa). These DRGs also exhibited highest gene and protein expression of proliferation marker Epha4, Ntn4, Sema3D and differentiation marker Unc5B. Our study revealed the mechanism of how material stiffness affects DRG proliferation and differentiation. It will also provide theoretical basis and evidence for the design and development of nerve graft with better repair performance.",

keywords = "Dorsal root ganglion, Gene and protein expression, Polyacrylamide, Stiffness",

author = "Liling Zhang and Qi Han and Shiyu Chen and Di Suo and Luzhong Zhang and Guicai Li and Xin Zhao and Yumin Yang",

note = "Funding Information: The authors gratefully acknowledge the financial support of the National Natural Science Foundation of China (31830028, 31771054, 11702233), Natural Key Science Research Program of Jiangsu Education Department (19KJA320006), The Open Project of Key Laboratory of Organ Regeneration and Transplantation, Ministry of Education (2020JC08), Qinglan Project of Jiangsu Province (2018), Large Instruments Open Foundation of Nantong University (KFJN2075). Funding Information: The authors gratefully acknowledge the financial support of the National Natural Science Foundation of China ( 31830028 , 31771054 , 11702233 ), Natural Key Science Research Program of Jiangsu Education Department ( 19KJA320006 ), The Open Project of Key Laboratory of Organ Regeneration and Transplantation, Ministry of Education ( 2020JC08 ), Qinglan Project of Jiangsu Province (2018) , Large Instruments Open Foundation of Nantong University ( KFJN2075 ). Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

year = "2021",

month = mar,

doi = "10.1016/j.colsurfb.2020.111503",

language = "English",

volume = "199",

journal = "Colloids and Surfaces B: Biointerfaces",

issn = "0927-7765",

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T1 - Soft hydrogel promotes dorsal root ganglion by upregulating gene expression of Ntn4 and Unc5B

AU - Zhang, Liling

AU - Han, Qi

AU - Chen, Shiyu

AU - Suo, Di

AU - Zhang, Luzhong

AU - Li, Guicai

AU - Zhao, Xin

AU - Yang, Yumin

N1 - Funding Information: The authors gratefully acknowledge the financial support of the National Natural Science Foundation of China (31830028, 31771054, 11702233), Natural Key Science Research Program of Jiangsu Education Department (19KJA320006), The Open Project of Key Laboratory of Organ Regeneration and Transplantation, Ministry of Education (2020JC08), Qinglan Project of Jiangsu Province (2018), Large Instruments Open Foundation of Nantong University (KFJN2075). Funding Information: The authors gratefully acknowledge the financial support of the National Natural Science Foundation of China ( 31830028 , 31771054 , 11702233 ), Natural Key Science Research Program of Jiangsu Education Department ( 19KJA320006 ), The Open Project of Key Laboratory of Organ Regeneration and Transplantation, Ministry of Education ( 2020JC08 ), Qinglan Project of Jiangsu Province (2018) , Large Instruments Open Foundation of Nantong University ( KFJN2075 ). Publisher Copyright: © 2020 Elsevier B.V.

PY - 2021/3

Y1 - 2021/3

N2 - Mechanical property is an important factor of cellular microenvironment for neural tissue regeneration. In this study, polyacrylamide (PAM) hydrogels with systematically varying elastic modulus were prepared using in situ radical polymerization. We found that the hydrogel was biocompatible, and the length of dorsal root ganglion (DRG)'s axon and cell density were optimal on the hydrogels with elastic modulus of 5.1 kPa (among hydrogels with elastic modulus between 3.6 kPa and 16.5 kPa). These DRGs also exhibited highest gene and protein expression of proliferation marker Epha4, Ntn4, Sema3D and differentiation marker Unc5B. Our study revealed the mechanism of how material stiffness affects DRG proliferation and differentiation. It will also provide theoretical basis and evidence for the design and development of nerve graft with better repair performance.

AB - Mechanical property is an important factor of cellular microenvironment for neural tissue regeneration. In this study, polyacrylamide (PAM) hydrogels with systematically varying elastic modulus were prepared using in situ radical polymerization. We found that the hydrogel was biocompatible, and the length of dorsal root ganglion (DRG)'s axon and cell density were optimal on the hydrogels with elastic modulus of 5.1 kPa (among hydrogels with elastic modulus between 3.6 kPa and 16.5 kPa). These DRGs also exhibited highest gene and protein expression of proliferation marker Epha4, Ntn4, Sema3D and differentiation marker Unc5B. Our study revealed the mechanism of how material stiffness affects DRG proliferation and differentiation. It will also provide theoretical basis and evidence for the design and development of nerve graft with better repair performance.

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KW - Gene and protein expression

KW - Polyacrylamide

KW - Stiffness

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JO - Colloids and Surfaces B: Biointerfaces

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Soft hydrogel promotes dorsal root ganglion by upregulating gene expression of Ntn4 and Unc5B

Abstract

Keywords

ASJC Scopus subject areas

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