Single strand conformation polymorphism (SSCP) analysis

Kim Hung Leung, Shea Ping Yip

Research output: Chapter in book / Conference proceedingChapter in an edited book (as author)Academic researchpeer-review


Variations in DNA sequences underlie the differences among different members of the same species and also between different species. DNA sequence variations are usually known as polymorphisms if the commonest allele is less than 0.99 in a given population (1). DNA polymorphisms are widespread in many different species, particularly in humans (2,3). Examples include single nucleotide polymorphisms (SNPs), microsatellites, minisatellites, small insertions/ deletions, and large insertions/deletions. DNA polymorphisms may not have any phenotypic effect at the protein level or at the level of the whole organism. On the other hand, they are usually called disease-causing or pathogenic mutations if they cause a change in the phenotype and results in a disease status. The frequencies of individual mutations are usually not high because of selection pressure against such less favorable base changes. It is thus important to study DNA sequence variations in various branches of biological sciences.
Original languageEnglish
Title of host publicationMolecular Biomethods Handbook
Subtitle of host publicationSecond Edition
PublisherHumana Press
Number of pages15
ISBN (Print)9781603273701
Publication statusPublished - 1 Dec 2008

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology


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